英文名称:EcoR I
其他名称:限制性内切酶EcoR I
CAS号:80498-17-5
级别:BR
来源:Recombinant E. coli strain that carries the cloned ecoRIR gene from Escherichia coli.
保存条件:10 mM potassium phosphate (pH 7.4 at 25°C), 300 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.2 mg/mL BSA, 0.15% Triton X-100 and 50% glycerol.
缓冲液Optimal buffer(10X):500 mM Tris-HCl (pH 7.5), 100 mM MgCl2, 1000 mM NaCl, 0.2% Triton X-100, 1 mg/mL BSA.
Ligations:After 10-fold over digestion with enzyme 95% of the DNA fragments can be ligated and recut.
Methylation Effects:Not dam methylation-sensitive, not dcm methylation-sensitive. CpG methylation-sensitive
Campatible Ends:Xap I, Mun I, Tas I, Acs I.
使用说明:High enzyme concentration and using of nonoptimal buffer may result in star activity. Do not use BSA for long incubation. To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
性状(以下信息仅供参考):液体
用途:本品仅供科研,不得用于其它用途。
保存:-20℃