产品货号 :  mlR13100

英文名称 :  EPS15R

中文名称 :  表皮生长因子受体底物15抗体

别    名 :  epidermal growth factor receptor pathway substrate 15- ike 1; Epidermal growth factor receptor substrate 15 like 1; epidermal growth factor receptor substrate EPS15R; Eps15 related protein; eps15R;EP15R_HUMAN.  

研究领域 :  细胞生物  生长因子和激素  激酶和磷酸酶  表观遗传学  

抗体来源 :  Rabbit

克隆类型 :  Polyclonal

交叉反应 :   Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,

产品应用 :  WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  94kDa

细胞定位 :  细胞核 细胞膜

性    状 :  Lyophilized or Liquid

浓    度 :  1mg/ml

免 疫 原 :  KLH conjugated synthetic peptide derived from human EPS15R:275-380/864

亚    型 :  IgG

纯化方法 :  affinity purified by Protein A

储 存 液 :  0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件 :  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed :  PubMed

产品介绍 :   Elucidation of the mechanism by which receptor tyrosine kinases (RTKs) modulate cellular physiology in response to stimuli is critical to the understanding of growth regulation, as miscues in RTK signaling can result in cellular transformation events that may ultimately lead to cancer. Eps15 (EGF-receptor pathway substrate 15) is an EGF receptor substrate that become become tyrosine phosphorylated subsequent to EGF stimulation. Over expression of Eps15 in NIH/3T3 cells causes cellular transformation, implying involvement of Eps15 in the regulation of cell proliferation. Eps15R (Eps15-related), also known as Eps15L1 (epidermal growth factor receptor substrate 15-like 1), is an 864 amino acid protein that contains one EF-hand domain and three EH domains. Localized to a variety of places within the cell, including the nucleus, cytoplasm and membrane-coated pits, Eps15R is thought to function as a component of clathrin-coated pits. Like Eps15, Eps15R may play an important role in receptor-mediated endocytosis.

Function:

EPS15 and EPS15R are substrates of the epidermal growth factor (EGF) receptor kinase, which are characterized by their ability to bind to the clathrin adaptor protein complex adaptor protein 2, and the presence of a protein:protein interaction domain, the EH domain. Evidence suggests that EPS15 and EPS15R are involved in endocytosis. The major difference between these two related proteins is that EPS15R is also found in the nucleus, whereas EPS15 is excluded from this compartment at steady state.

Subunit:

Interacts with EPS15, AGFG1/HRB and AGFG2/HRBL. Associates with the clathrin-associated adapter protein complex 2 (AP-2) (By similarity). Interacts with FCHO1. Interacts with FCHO2. Interacts (via EH domains) with DAB2.

Subcellular Location:

Cell Membrane and Nuclear.

Post-translational modifications:

Phosphorylated on tyrosine residues by EGFR (By similarity).

Similarity:

Contains 1 EF-hand domain.

Contains 3 EH domains.

SWISS:

Q9UBC2

Gene ID:

58513

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片