产品货号 :  mlR18995

英文名称 :  Naca

中文名称 :  初生多肽相关复合物亚单位α，肌肉特异型蛋白抗体

别    名 :  NACAM_HUMAN; Nascent polypeptide-associated complex subunit alpha, muscle-specific form; Alpha-NAC, muscle-specific form; skNAC; skNAC; Gm1878; AL022831; AL024382; mKIAA0363.  

研究领域 :  细胞生物  免疫学  

抗体来源 :  Rabbit

克隆类型 :  Polyclonal

交叉反应 :  Human, Mouse, Dog, Sheep,

产品应用 :  ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  229kDa

细胞定位 :  细胞核 细胞浆

性    状 :  Lyophilized or Liquid

浓    度 :  1mg/ml

免 疫 原 :  KLH conjugated synthetic peptide derived from human Alpha-NAC, muscle-specific form:121-215/215

亚    型 :  IgG

纯化方法 :  affinity purified by Protein A

储 存 液 :  0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件 :  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed :  PubMed

产品介绍 :  NACA1, NACA2 and NACAP1 are members of the nascent polypeptide-associated complex (NAC) α subunit family that participate in preventing inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). As nascent polypeptide chains emerge from the ribosome, NACA proteins bind to these chains and block their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. Members of the α-NAC subunit family have sequence similarities with transcription-regulating proteins and are suggested to function as transcriptional coactivators potentiating c-Jun-mediated transcription. Most NACA proteins localize to both nucleus as well as cytoplasm, and contain NAC-A/B (NAC-α/β) and UBA (ubiquitin-associated) domains. The UBA domain is associated with proteins involved in the ubiquitin-proteasome pathway for protein degradation.

Function:

Cardiac- and muscle-specific transcription factor. May act to regulate the expression of genes involved in the development of myotubes. Plays a critical role in ventricular cardiomyocyte expansion and regulates postnatal skeletal muscle growth and regeneration. Involved in the organized assembly of thick and thin filaments of myofibril sarcomeres.

Subunit:

Interacts (via PXLXP motif) with the muscle-restricted histone methyltransferase SMYD1 (via MYND-type zinc finger).

Subcellular Location:

Cytoplasm. Nucleus.

Tissue Specificity:

Specifically expressed in heart and skeletal muscle: it is present in differentiated myotubes but not in myoblasts.

Post-translational modifications:

Phosphorylation of Ser-2015 by ILK during cell adhesion may promote nuclear localization. Phosphorylation of Thr-2131 by GSK3B may promote proteasome mediated degradation.

Similarity:

Contains 1 NAC-A/B (NAC-alpha/beta) domain.

Contains 1 UBA domain.

SWISS:

E9PAV3

Gene ID:

17938

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.