产品货号 : mlR6997
英文名称 : SAP62
中文名称 : 剪接体相关蛋白62抗体
别 名 : PRPF11; SF3a66; SAP 62; SAP-62; Spliceosome-associated protein 62; splicing factor 3a subunit 2; SF3A2_HUMAN.
研究领域 : 细胞生物 表观遗传学
抗体来源 : Rabbit
克隆类型 : Polyclonal
交叉反应 : Human, Mouse, Rat, Chicken, Dog, Cow,
产品应用 : WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 : 49kDa
细胞定位 : 细胞核
性 状 : Lyophilized or Liquid
浓 度 : 1mg/ml
免 疫 原 : KLH conjugated synthetic peptide derived from human SAP62:65-160/464
亚 型 : IgG
纯化方法 : affinity purified by Protein A
储 存 液 : 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 : Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed : PubMed
产品介绍 : SAP 62, also known as SF3A2 (Splicing factor 3A subunit 2), PRP11, PRPF11 or SF3a66, is a 464 amino acid protein that contains one matrin-type zinc finger and belongs to the SF3A2 family. Localized to the nucleus, SAP 62 is a subunit of the SF3A splicing factor, a heterotrimeric complex comprised of three subunits that act in tandem to mediate the binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. The SF3A complex is necessary for the conversion of 15S U2 snRNP into the active 17S protein that performs directly in pre-mRNA splicing events. Functioning as the second subunit of the complex, SAP 62 interacts with subunit 1 (SAP 114) via its N-terminus while simultaneously binding to 15S U2 snRNP via its zinc finger domain. In addition to its role in RNA splicing, SAP 62 is thought to act independently as a microtubule-binding protein.
Function:
Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex.
Subunit:
Component of splicing factor SF3A which is composed of three subunits; SF3A3/SAP61, SF3A2/SAP62, SF3A1/SAP114. SF3A associates with the splicing factor SF3B and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Identified in the spliceosome C complex. Interacts with HTATSF1.
Subcellular Location:
Nuclear
Tissue Specificity:
Component of splicing factor SF3A which is composed of three subunits; SF3A3/SAP61, SF3A2/SAP62, SF3A1/SAP114. SF3A associates with the splicing factor SF3B and a 12S RNA unit to form the U2 small nuclear ribonucleoproteins complex (U2 snRNP). Identified in the spliceosome C complex. Interacts with HTATSF1.
Similarity:
Belongs to the SF3A2 family.
Contains 1 matrin-type zinc finger.
SWISS:
Q15428
Gene ID:
8175
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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