产品货号 :  mlR30078

英文名称 :  human CD33-APC

中文名称 :  APC标记小鼠抗人CD33单克隆抗体

别    名 :  CD33_HUMAN; CD 33; CD33 antigen; CD33 molecule; FLJ00391; gp67 ; Myeloid cell surface antigen CD33; Myeloid cell surface antigen CD33 precursor; p67 ; Sialic acid binding Ig like lectin 3; Sialic acid binding immunoglobulin like lectin 3; SIGLEC3; Siglec-3; myeloid cell surface antigen CD33 isoform 1 precursor.  

研究领域 :  免疫学  干细胞  细胞表面分子  

抗体来源 :  Mouse

克隆类型 :  Monoclonal

克 隆 号 :  HI33a

交叉反应  :  Human,

产品应用 :  Flow-Cyt=20ul/Test  

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  40/37kDa

细胞定位 :  细胞膜

性    状 :   Liquid

亚    型 :  IgG2a

纯化方法 :  affinity purified by Protein G

储 存 液 :  0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件 :  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed :  PubMed

产品介绍 :   CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias.

Function:

Putative adhesion molecule of myelomonocytic-derived cells that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Induces apoptosis in acute myeloid leukemia (in vitro).

Subunit:

Interacts with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation.

Subcellular Location:

Cell membrane; Single-pass type I membrane protein.

Tissue Specificity:

Monocytic/myeloid lineage cells.

Post-translational modifications:

Phosphorylation of Tyr-340 is involved in binding to PTPN6 and PTPN11.

Phosphorylation of Tyr-358 is involved in binding to PTPN6.

Similarity:

Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.

Contains 1 Ig-like C2-type (immunoglobulin-like) domain.

Contains 1 Ig-like V-type (immunoglobulin-like) domain.

SWISS:

P20138

Gene ID:

945

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片