产品货号 :  mlR7110

英文名称 :  DOCK1

中文名称 :  细胞质分裂付出蛋白1抗体

别    名 :  180 kDa protein downstream of CRK; Ced5; Dedicator of Cytokinesis 1; Dedicator of cytokinesis protein 1; DOCK 1; DOCK-1; DOCK 180; DOCK1; DOCK1_HUMAN; DOCK180.  

研究领域 :  肿瘤  细胞生物  信号转导  细胞凋亡  细胞自噬  G蛋白信号  

抗体来源 :  Rabbit

克隆类型 :  Polyclonal

交叉反应 :   Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Sheep,

产品应用 :  ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  215kDa

细胞定位 :  细胞浆 细胞膜

性    状 :  Lyophilized or Liquid

浓    度 :  1mg/ml

免 疫 原 :  KLH conjugated synthetic peptide derived from human DOCK1:465-550/1865

亚    型 :  IgG

纯化方法 :  affinity purified by Protein A

储 存 液 :  0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件 :  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed :  PubMed

产品介绍 :   Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1.

Tissue specificity: Highly expressed in placenta, lung, kidney, pancreas and ovary. Expressed at intermediate level in thymus, testes and colon.

Function:

Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Functions as a guanine nucleotide exchange factor (GEF), which activates Rac Rho small GTPases by exchanging bound GDP for free GTP. Its GEF activity may be enhanced by ELMO1.

Subunit:

Interacts with the SH3 domains of CRK and NCK2 via multiple sites. Interacts with nucleotide-free RAC1 via its DHR-2 domain. Interacts with ELMO1, ELMO2 and probably ELMO3 via its SH3 domain. Interacts with RAC1 and BAI1.

Subcellular Location:

Cytoplasm (Probable). Membrane (Probable). Note=Recruited to membranes via its interaction with phosphatidylinositol 3,4,5-trisphosphate (Probable).

Tissue Specificity:

Highly expressed in placenta, lung, kidney, pancreas and ovary. Expressed at intermediate level in thymus, testes and colon.

Similarity:

Belongs to the DOCK family.

Contains 1 DHR-1 domain.

Contains 1 DHR-2 domain.

Contains 1 SH3 domain.

SWISS:

Q14185

Gene ID:

1793

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

                                    乳腺癌转移的新标记物DOCK1

    乳腺癌是一种常常发生转移的癌症。将近90%的乳腺癌患者都因为癌症扩散而死，因此，理解这一危险过程的细胞和分子基础机制是十分重要的。

    近日，加拿大蒙特利尔临床研究所（Institut de recherches cliniques de Montreal, IRCM）的科学家在乳腺癌研究方面取得了一个重要的发现，他们确定了，一种名为DOCK1的蛋白质可以作为潜在的靶标用于减少乳腺癌患者的癌转移。相关研究结果刊登在了近期出版的《PNAS》上。

    研究人员在乳腺癌治疗方面尽管已经取得了重要的进展，但是对于癌症扩散的机制却知之甚少。科学家正在试图确定能够调节癌转移过程的蛋白质，因为它们或许可以作为新的药物靶标，并与现有的方法相结合促进治疗的效果。

    在这项研究中，2个主要的癌症亚型——HER-2+型和Basal-like型，很容易转移和复发，并最终导致患者的低生存率。该调查以HER-2+(人类表皮生长因子受体2)型为研究对象， 这代表了大约25%的乳腺癌病例。 HER2阳性肿瘤比其它类型的肿瘤更倾向于快速的发展和扩散。

    研究人员解释到，通过研究HER2+乳腺癌小鼠模型，确定了DOCK1蛋白质是癌转移的一个重要的调控因子。当去除了小鼠中的这一蛋白质后，乳腺癌的肺转移大大减少了。此外，研究发现DOCK1蛋白质有助于肿瘤的生长。

    为了证明DOCK1的表达与乳腺癌预后之间的关系，研究人员对包含患者基因信息的几个数据库进行了分析。结果发现，在HER-2+型或Basal-like型乳腺癌中，高水平的DOCK1与较差的预后以及疾病的复发有关。

产品图片