产品货号 :  mlR8852

英文名称 :  d-myc

中文名称 :  d-myc蛋白抗体

别    名 :  dMyc1; MYC_DROME; Myc protein; d-Myc1; d-myc.  

研究领域 :  细胞生物  免疫学  

抗体来源 :  Rabbit

克隆类型 :  Polyclonal

交叉反应 :  Firefly, fruit fly

产品应用 :  WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  79kDa

细胞定位 :  细胞核

性    状 :  Lyophilized or Liquid

浓    度 :  1mg/ml

免 疫 原 :  KLH conjugated synthetic peptide derived from fruit fly d-myc:121-220/717

亚    型 :  IgG

纯化方法 :  affinity purified by Protein A

储 存 液 :  0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件 :  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed :  PubMed

产品介绍 :   Drosophila melanogaster is a proven and effective model for studying developmental and cellular processes common to higher eukaryotes. Approximately 13,600 genes have been elucidated from more than 120 megabases of euchromatin, and they are organized among the chromosomes 2, 3, 4, X and Y, with the Y chromosome being predominately heterochromatic (1). Drosophila genes can be categorized based on the type of protein they encode and are represented by six major classifications, which include intracellular signaling proteins, transmembrane proteins, RNA binding proteins, secreted factors, transcription regulators (basic helix-loop-helix, homeodomain containing, zinc finger containing, and chromatin associated) or other functional proteins (2). Many of the proteins in Drosophila are structurally and functionally similar across species, as are the pathways involved in transducing intracellular signaling. Among these proteins, myc (d-myc, dmyc1) is a transcription factor that links patterning signals to cell division by regulating events coordinating cellular growth and metabolism (3-7).

Function:

Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence CAC[GA]TG. Seems to activate the transcription of growth-related genes; required for cellular proliferation and growth. Inhibits the demethylase activity of Lid.

Subunit:

Heterodimer with another bHLH proteins. Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with Max. Interacts with lid. Part of a complex containing lid, dm and ash2. Component of a complex with pont and rept.

Subcellular Location:

Nucleus.

Tissue Specificity:

Low levels detected throughout embryo before cellular blastoderm formation, particularly concentrated in pole plasm. Zygotic expression detected during cellular blastoderm stage in endodermal anlagen of anterior and posterior midgut at both poles. After gastrulation, expression detected in invaginating ventral furrow of mesoderm. Continued expression in anterior and posterior midgut and mesoderm during germband extension. During late germ-band retraction, expression remains detectable in fusing midgut and presumed developing somatic musculature.

Similarity:

Contains 1 bHLH (basic helix-loop-helix) domain.

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.