产品货号 : mlR14357
英文名称 : DNA Primase
中文名称 : DNA引物酶抗体
别 名 : dJ422B11.1.1; DNA primase 58kDa subunit; DNA primase large subunit; DNA primase p58; DNA primase subunit p58; p58; Polypeptide 2A p58; PRIM2; PRIM2A; PRI2_HUMAN; Primase DNA polypeptide 2 (58kDa); Primase polpeptide 2A (58kD); Primase polpeptide 2A 58kDa.
研究领域 : 细胞生物 结合蛋白 表观遗传学
抗体来源 : Rabbit
克隆类型 : Polyclonal
交叉反应 : Human, Mouse, Rat, Pig,
产品应用 : WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 : 59kDa
细胞定位 : 细胞核
性 状 : Lyophilized or Liquid
浓 度 : 1mg/ml
免 疫 原 : KLH conjugated synthetic peptide derived from human DNA Primase:51-150/509
亚 型 : IgG
纯化方法 : affinity purified by Protein A
储 存 液 : 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 : Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed : PubMed
产品介绍 : This gene encodes the 58 kilodalton subunit of DNA primase, an enzyme that plays a key role in the replication of DNA. The encoded protein forms a heterodimer with a 49 kilodalton subunit. This heterodimer functions as a DNA-directed RNA polymerase to synthesize small RNA primers that are used to create Okazaki fragments on the lagging strand of the DNA. [provided by RefSeq, Sep 2013]
Function:
DNA primase synthesizes small ribonucleotide primers for the initiation of DNA replication. The eukaryotic primase is a heterodimer of a small and a large subunit, p49 and p58 respectively. These two subunits purify as a complex tightly bound to DNA polymerase alpha. This tight association implicates polymerase alpha as the lagging-strand DNA polymerase in replication. Uniquely, primases are able to synthesize nucleotides de novo on a template by the formation of an initial dinucleotide. DNA primase initiates synthesis with a triphosphate purine moiety at the 5'-end. After synthesis of 7-10 ribonucleotides, the primer template is translocated intramolecularly to the active site of the DNA polymerase alpha subunit. The p49 subunit of DNA primase contains the catalytic active site. The human primase subunits are 90% identical in amino acid sequence to the mouse homologs.
Subunit:
Heterodimer of a small subunit and a large subunit.
Similarity:
Belongs to the eukaryotic-type primase large subunit family.
SWISS:
P49643
Gene ID:
5558
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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