产品货号 :  mlR51210 

英文名称 :  CYP1A1 

中文名称 :  小鼠抗细胞色素P450 1A1单克隆抗体 

别    名 :  Cytochrome p450 1A1; AHH; AHRR; CP11; CYP1; P1-450; P450-C; P450DX; AHH; AHRR; Aryl hydrocarbon hydroxylase; CYP 1; CYP1A1; CYPIA1; Cytochrome P1-450; Flavoprotein-linked monooxygenase; Microsomal monooxygenase; P1 450; P450 C; P450 form 6; P450 P1; Xenobiotic monooxygenase.   

研究领域 :  细胞生物  免疫学  合成与降解   

抗体来源 :  Mouse

克隆类型 :  Monoclonal 

克 隆 号 :  10G3 

交叉反应 :   Human, Mouse, 

产品应用 :  WB=1:500-2000 ELISA=1:500-1000  

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user. 

分 子 量 :  58kDa 

细胞定位 :  细胞浆 细胞膜 

性    状 :  Lyophilized or Liquid 

浓    度 :  1mg/ml

免 疫 原 :  KLH conjugated synthetic peptide derived from human CYP1A1:

亚    型 :  IgG1

纯化方法 :  affinity purified by Protein G

储 存 液 :  0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件 :  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed :  PubMed

产品介绍  :  The Cytochrome P450 (P450) superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several cofactors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). As well as fatty acid metabolism, P450s also metabolize many drugs and toxins. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin. 

Function:

Mediates dioxin toxicity and is involved in regulation of cell growth and differentiation. Represses the transcription activity of AHR by competing with this transcription factor for heterodimer formation with the ARNT and subsequently binding to the xenobiotic response element (XRE) sequence present in the promoter regulatory region of variety of genes. Represses CYP1A1 by binding the XRE sequence and recruiting ANKRA2, HDAC4 and/or HDAC5. Autoregulates its expression by associating with its own XRE site.

Subcellular Location:

Cytochrome P450 1A1: Cytoplasm.

Cytochrome P450MT2A: Endoplasmic reticulum membrane. Mitochondrion membrane. Microsome membrane.

Cytochrome P450MT2B: Endoplasmic reticulum. Mitochondrion.

Tissue Specificity:

Highly expressed in testis, lung, ovary, spleen and pancreas. Highly expressed in mononuclear cells (MNCs) from umbilical cord blood. Isoform 3 is highly expressed in lung, kidney, spleen and thymus. Down-regulated malignant tissue from different anatomical origins, including colon, breast, lung, stomach, cervix, and ovary.

Similarity:

Belongs to the cytochrome P450 family 

SWISS:

A9YTQ3

Gene ID:

57491

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.