产品货号 :  mlR19026

英文名称 :  NASP

中文名称 :  核自身抗原精子蛋白NASP抗体

别   名 :  5033430J04Rik; AI131596; AI317140; D4Ertd767e; DKFZp547F162; Epcs32; FLB7527; FLJ31599; FLJ35510; MGC19722; MGC20372; MGC2297; MGC93869; NASP; NASP_HUMAN; nuclear autoantigenic sperm protein (histone binding); Nuclear autoantigenic sperm protein; PRO1999; RP23 233B9.9.  

研究领域 :  肿瘤  细胞生物  免疫学  染色质和核信号  转录调节因子  表观遗传学  

抗体来源 :  Rabbit

克隆类型 :  Polyclonal

交叉反应 :   Human, Mouse, Rat, Cow, Horse, Rabbit, Sheep,

产品应用 :  WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  85kDa

细胞定位 :  细胞核 细胞浆

性    状 :  Lyophilized or Liquid

浓    度 :  1mg/ml

免 疫 原 :  KLH conjugated synthetic peptide derived from human NASP:2-100/788

亚    型 :  IgG

纯化方法 :  affinity purified by Protein A

储 存 液 :  0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件 :  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed :  PubMed

产品介绍 :   This gene encodes a H1 histone binding protein that is involved in transporting histones into the nucleus of dividing cells. Multiple isoforms are encoded by transcript variants of this gene. The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle. The testicular form is expressed in embryonic tissues, tumor cells, and the testis. In male germ cells, this protein is localized to the cytoplasm of primary spermatocytes, the nucleus of spermatids, and the periacrosomal region of mature spermatozoa. [provided by RefSeq, Jul 2008]

Function:

Required for DNA replication, normal cell cycle progression and cell proliferation. Forms a cytoplasmic complex with HSP90 and H1 linker histones and stimulates HSP90 ATPase activity. NASP and H1 histone are subsequently released from the complex and translocate to the nucleus where the histone is released for binding to DNA.

Subcellular Location:

Cytoplasm. Nucleus.

Tissue Specificity:

Isoform 1 is testis- and sperm-specific.

Post-translational modifications:

Phosphorylated upon DNA damage, probably by ATM or ATR.

Similarity:

Belongs to the NASP family.

Contains 3 TPR repeats.

SWISS:

P49321

Gene ID:

4678

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片