产品货号 :  mlR1687

英文名称 :  DAPK1

中文名称 :  凋亡相关蛋白激酶1抗体

别    名 :  DAP Kinase 1; DAPK1; DAK1; DAP K1; DAPK 1; DAPK; DAPK1; Death Associated Protein Kinase 1; Death Associated Protein Kinase 1; Death-associated protein kinase 1; DKFZp781I035; DAPK1_HUMAN.  

研究领域 :  肿瘤  细胞生物  信号转导  细胞凋亡  激酶和磷酸酶  

抗体来源 :  Rabbit

克隆类型 :  Polyclonal

交叉反应  :  Human, Mouse, Rat,

产品应用 :  WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  160kDa

细胞定位 :  细胞浆

性    状 :  Lyophilized or Liquid

浓    度 :  1mg/ml

免 疫 原 :  KLH conjugated synthetic peptide derived from human DAPK1:1331-1430/1430

亚    型 :  IgG

纯化方法 :  affinity purified by Protein A

储 存 液 :  0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件 :  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed :  PubMed

产品介绍 :   DAPK1 expression is frequently lost in human carcinomas and B-cell leukemia, and lower levels of expression correlates with high rates of metastasis. The loss of DAPK expression provides a link between suppression of apoptosis and metastasis. DAPK1 is thought be involved in an early apoptotic checkpoint which eliminates premalignant cells from cancer formation. Studies in bladder cancer patients have also shown that hypermethylation of DAPK1 correlates to high recurrence rates and thus DAPK1 may be used as a prognostic marker. DAPK1 is also reportedly a molecular regulator of neuronal death in epilepsy.

Function:

Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.

Subunit:

Interacts with KLHL20. Interacts (via death domain) with MAPK1 and MAPK3. Interacts with MAP1B (via N-terminus). Interacts (via death domain) with UNC5B (via death domain). Interacts with PRKD1 in an oxidative stress-regulated manner. Interacts with PIN1, PDCD6, BECN1, GRINB, TSC2 and STX1A. Interacts (via kinase domain) with DAPK3 (via kinase domain).

Subcellular Location:

Cytoplasm. Colocalizes with the actin filament system.

Tissue Specificity:

Isoform 2 is expressed in normal intestinal tissue as well as in colorectal carcinomas.

Post-translational modifications:

Ubiquitinated by the BCR(KLHL20) E3 ubiquitin ligase complex, leading to its degradation by the proteasome.

Similarity:

Belongs to the protein kinase superfamily.

CAMK Ser/Thr protein kinase family.

DAP kinase subfamily.

Contains 10 ANK repeats.

Contains 1 death domain.

Contains 1 protein kinase domain.

SWISS:

P53355

Gene ID:

1612

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

凋亡相关蛋白激酶1(DAPK1)又称：一般性白血病的基因，也是一种肿瘤抑制基因，广泛存在于各种细胞的凋亡系统中，该基因正常情况下有助于触发细胞在发生癌变前就死亡。但异常的DNA甲基化作用会关闭控制细胞生长的DAPK1基因，从而造成肿瘤生长。