产品货号 : mlR3542
英文名称 : DEM1/PNK
中文名称 : 多聚合苷酸激酶3磷酸化酶抗体
别 名 : PNK1; Bifunctional polynucleotide phosphatase/kinase; DEM 1; DEM1; DNA 5' kinase/3' phosphatase; PNK 1; PNK; PNK1; PNKP; Polynucleotide 5' hydroxyl kinase; Polynucleotide kinase 3 prime phosphatase; Polynucleotide kinase 3' phosphatase; Polynucleotide Kinase; PNKP_HUMAN.
研究领域 : 肿瘤 细胞生物 免疫学 信号转导 细胞凋亡 转录调节因子 激酶和磷酸酶
抗体来源 : Rabbit
克隆类型 : Polyclonal
交叉反应 : Human, Mouse, Rat, Dog, Cow, Sheep,
产品应用 : ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 : 57kDa
细胞定位 : 细胞核
性 状 : Lyophilized or Liquid
浓 度 : 1mg/ml
免 疫 原 : KLH conjugated synthetic peptide derived from human PNK1/PNKP:51-160/521
亚 型 : IgG
纯化方法 : affinity purified by Protein A
储 存 液 : 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 : Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed : PubMed
产品介绍 : Mammalian PNK catalyzes the phosphorylation of DNA at 5'-hydroxyl termini and can dephosphorylate its 3'-phosphate termini. It plays an important function in DNA repair following ionizing radiation or oxidative damage. PNK has been reported to participate in the repair of DNA-double strand breaks via PARP-1-dependent nonhomologous end-joining.
Function:
Plays a key role in the repair of DNA damage,functioning as part of both the non-homologous end-joining (NHEJ)and base excision repair (BER) pathways. Through its two catalyticactivities, PNK ensures that DNA termini are compatible withextension and ligation by either removing 3'-phosphates from, or byphosphorylating 5'-hydroxyl groups on, the ribose sugar of the DNAbackbone.
Subunit:
Monomer (By similarity).
Subcellular Location:
Nucleus.
Tissue Specificity:
Expressed in many tissues with highestexpression in spleen and testis, and lowest expression in smallintestine (PubMed:10446192). Expressed in higher amount inpancreas, heart and kidney and at lower levels in brain, lung andliver (PubMed:10446193).
Post-translational modifications:
Phosphorylated upon DNA damage, probably by ATM or ATR.
DISEASE:
Defects in PNKP are the cause of epilepticencephalopathy, early infantile, type 10 (EIEE10) [MIM:613402]. Adisease characterized by microcephaly, infantile-onset seizures,severe intellectual disability and delayed motor milestones withabsent speech or only achieving a few words. Most patients alsohave behavioral problems with hyperactivity. Microcephaly isprogressive and without neuronal migration or structuralabnormalities, consistent with primary microcephaly.
Similarity:
In the N-terminal section; belongs to the DNA 3'phosphatase family.
Contains 1 FHA domain.
SWISS:
Q96T60
Gene ID:
11284
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
