产品货号 :  mlR20108

英文名称 :  HPV16 E4

中文名称 :  人乳头瘤病毒16型E4抗体

别    名 :  E4; Human Papilloma Virus; Human papillomavirus type 16 E4; Human papillomavirus type 16 E4; Protein E4; Human Papillomavirus 16 (E4); HPV16 E4; E4 protein (HPV16); VE4_HPV16; HPV16-E4; Probable protein E4.  

研究领域 :  细菌及病毒  

抗体来源 :  Rabbit

克隆类型 :  Polyclonal

交叉反应 :  HPV16

产品应用 :  ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  11kDa

细胞定位 :  细胞核 细胞浆

性    状 :  Lyophilized or Liquid

浓    度 :  1mg/ml

免 疫 原 :  KLH conjugated synthetic peptide derived from human HPV16 E4:41-95/95

亚    型 :  IgG

纯化方法 :  affinity purified by Protein A

储 存 液 :  0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件 :  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed :  PubMed

产品介绍 :  The papillomavirus E4 open reading frame (ORF) is contained within the E2 ORF, with the primary E4 gene-product (E1∧E4) being translated from a spliced mRNA that includes the E1 initiation codon and adjacent sequences. E4 is located centrally within the E2 gene, in a region that encodes the E2 protein′s flexible hinge domain. Although a number of minor E4 transcripts have been reported, it is the product of the abundant E1∧E4 mRNA that has been most extensively analysed.

Function:

Plays several roles to favor viral DNA replication including disruption of host intranuclear speckles (termed ND10 body) organization, cell cycle arrest at the G2 phase and inhibition of cellular DNA replication, by preventing loading of host replication licensing proteins MCM2 and MCM7 onto chromatin. Additionally, E4 is found in inclusion bodies within the cytoplasm associated with host SRPK1, a splicing factor regulator. Therefore, E4 may regulate host splicing factors in order to favor expression of late viral transcripts. Late in the infectious cycle, E4 may also act to diminish the integrity of the keratinocyte by disrupting the keratin cytoskeleton and inducing apoptosis through alteration of mitochondrial function to facilitate egress of the newly formed virions.

Subunit:

Assembles into oligomeric complexes. Interacts with host SRPK1; this interaction may favor expression of late viral transcripts. Interacts with host cytokeratin components KRT8 and KRT18.

Subcellular Location:

Host cytoplasm. Host nucleus.

Post-translational modifications:

Phosphorylated by host ERK. The phosphorylation triggers a structural change that enhances keratin binding and protein stability.

Similarity:

Belongs to the papillomaviridae E4 protein family.

Gene ID:

1489076

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.