产品货号 :  mlR23048

英文名称 :  Calpain 1

中文名称 :  钙蛋白酶1抗体

别    名 :  Calcium activated neutral proteinase 1; Calpain1; Calpain-1; Calpain 1 catalytic subunit; Calpain large polypeptide L1; Calpain mu type; CANP 1; CANP; CANP1; CANPL1; CAPN 1; CAPN1; Cell proliferation inducing gene 30 protein; Cell proliferation inducing protein 30; Micromolar calpain; muCANP; muCL; Ca2 activated neutral protease; Calcium activated neutral proteinase small subunit; Calcium dependent protease small subunit 1; Calcium-activated neutral proteinase 1; Calpain 1 (mu/I) large subunit; Calpain 1 catalytic subunit; Calpain 1 large subunit; Calpain Large Polypeptide L1; Calpain Large Polypeptide L1; Calpain mu type; Calpain mu-type; Calpain regulatory subunit; Calpain small subunit 1; Calpain-1 catalytic subunit; Calpain-1 large subunit; Calpain1; CAN1_HUMAN.  

研究领域 :  信号转导  细胞凋亡  激酶和磷酸酶  

抗体来源 :  Rabbit

克隆类型 :  Polyclonal

交叉反应 :   Human, Mouse, Rat,

产品应用 :  ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  78kDa

细胞定位 :  细胞浆 细胞膜

性    状 :  Lyophilized or Liquid

浓    度 :  1mg/ml

免 疫 原 :  KLH conjugated synthetic peptide derived from human Calpain 1:521-620/713

亚    型 :  IgG

纯化方法 :  affinity purified by Protein A

储 存 液 :  0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件 :  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed :  PubMed

产品介绍 :   The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Function:

Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.

Subunit:

Forms a heterodimer with a small (regulatory) subunit (CAPNS1).

Subcellular Location:

Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.

Tissue Specificity:

Ubiquitous.

Post-translational modifications:

Undergoes calcium-induced successive autoproteolytic cleavages that generate a membrane-bound 78 kDa active form and an intracellular 75 kDa active form. Calpastatin reduces with high efficiency the transition from 78 kDa to 75 kDa calpain forms.

Similarity:

Belongs to the peptidase C2 family. Contains 1 calpain catalytic domain. Contains 4 EF-hand domains.

SWISS:

P07384

Gene ID:

823

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

钙蛋白酶(calpain)是一族钙离子依赖性的、水解半胱氨酸的蛋白酶,广泛存在于全身各脏器组织中。钙蛋白酶参与不同细胞过程,包括细胞骨架和细胞膜附着的构型重建、不同的信号转导途径和细胞凋亡等.

产品图片