产品货号 :  mlR3550

英文名称 :  mlRMyogenin

中文名称 :  mlR肌细胞生成素抗体

别    名 :  mlRMYOG; bHLHc3; cb553; MYF 4; MYF4; Myf-4; MYOG; Myogenic factor 4; Myogenic factor 4 myogenin; MYOGENIN; Myogenin myogenin factor 4; OTTHUMP00000039094; Class C basic helix-loop-helix protein 3; MYOG_HUMAN.  

研究领域 :  mlR心血管  激酶和磷酸酶  结合蛋白  细胞表面分子  

抗体来源 :  mlRRabbit

克隆类型 :  mlRPolyclonal

交叉反应 :  mlR Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Rabbit, Sheep,

产品应用 :  mlRWB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  mlR25kDa

细胞定位 :  mlR细胞核

性    状 :  mlRLyophilized or Liquid

浓    度 :  mlR1mg/ml

免 疫 原 :  mlRKLH conjugated synthetic peptide derived from human MYOG/Myogenin:65-180/224

亚    型 :  mlRIgG

纯化方法 :  mlRaffinity purified by Protein A

储 存 液 :  mlR0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件 :  mlRStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed :  mlRPubMed

产品介绍 :   Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle. [provided by RefSeq, Jul 2008]

Function:

Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation, cell cycle exit and muscle atrophy. Essential for the development of functional embryonic skeletal fiber muscle differentiation. However is dispensable for postnatal skeletal muscle growth; phosphorylation by CAMK2G inhibits its transcriptional activity in respons to muscle activity. Required for the recruitment of the FACT complex to muscle-specific promoter regions, thus promoting gene expression initiation. During terminal myoblast differentiation, plays a role as a strong activator of transcription at loci with an open chromatin structure previously initiated by MYOD1. Together with MYF5 and MYOD1, co-occupies muscle-specific gene promoter core regions during myogenesis. Cooperates also with myocyte-specific enhancer factor MEF2D and BRG1-dependent recruitment of SWI/SNF chromatin-remodeling enzymes to alter chromatin structure at myogenic late gene promoters. Facilitates cell cycle exit during terminal muscle differentiation through the up-regulation of miR-20a expression, which in turn represses genes involved in cell cycle progression. Binds to the E-box containing (E1) promoter region of the miR-20a gene. Plays also a role in preventing reversal of muscle cell differentiation. Contributes to the atrophy-related gene expression in adult denervated muscles. Induces fibroblasts to differentiate into myoblasts.

Subunit:

Homodimer and heterodimer with E12; heterodimerization enhances MYOG DNA-binding and transcriptional activities. Interacts with SMARCA4/BRG1/BAF190A. Interacts (via C-terminus region) with SSRP1 and SUPT16H; the interaction is indicative of an interaction with the FACT complex.

Subcellular Location:

Nucleus. Note=Recruited to late myogenic gene promoter regulatory sequences with SMARCA4/BRG1/BAF190A and SWI/SNF chromatin-remodeling enzymes to promote chromatin-remodeling and transcription initiation in developing embryos.

Post-translational modifications:

Phosphorylated by CAMK2G on threonine and serine amino acids in a muscle activity-dependent manner. Phosphorylation of Thr-87 impairs both DNA-binding and trans-activation functions in contracting muscles.

Similarity:

Contains 1 bHLH (basic helix-loop-helix) domain.

SWISS:

P15173

Gene ID:

4656

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

MYOG是肌肉中运载氧的蛋白质，由153个氨基酸残基组成，一种小分子蛋白质，含有血红素，和血红蛋白同源，与氧的结合能力介于血红蛋白和细胞色素氧化酶之间，可帮助肌细胞将氧转运到线粒体。 肌细胞生成素在成肌分化过程中起着决定性的作用。在人体骨骼肌的胚胎发育过程中 ,肌细胞生成素伴随成肌过程而呈高水平表达。MYOG主要由肾脏代谢并排泄，当心肌或横纹肌有损伤时，肌红蛋白便释放入血中，血清中的肌红蛋白即可明显升高。