产品货号 :  mlR17648

英文名称 :  Spectrin beta 5

中文名称 :  血影蛋白抗体β5/spectrin β V抗体

别    名 :  Beta-V spectrin; brain 4; BSPECV; HUBSPECV; HUSPECV; non-erythroid beta chain 4; Spectrin; Spectrin beta chain; Spectrin beta chain, brain 4; Spectrin, non-erythroid beta chain 4; SPTBN4; SPTBN5; SPTN5_HUMAN.  

研究领域 :  细胞生物  信号转导  细胞骨架  

抗体来源 :  Rabbit

克隆类型 :  Polyclonal

交叉反应 :  Human,

产品应用 :  ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  417kDa

细胞定位 :  细胞浆

性    状 :  Lyophilized or Liquid

浓    度 :  1mg/ml

免 疫 原 :  KLH conjugated synthetic peptide derived from human Spectrin beta 5:1401-1500/3674

亚    型 :  IgG

纯化方法 :  affinity purified by Protein A

储 存 液 :  0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件 :  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed :  PubMed

产品介绍 :  Spectrin, an Actin binding protein that is a major component of the cytoskeletal superstructure of the erythrocyte plasma membrane, is essential in determining the properties of the membrane including its shape and deformability. Spectrins function as membrane organizers and stabilizers, composed of nonhomologous å and ∫ chains, which aggregate side-to-side in an antiparallel fashion to form dimers, tetramers, and higher polymers. The spectrin tetramers in erythrocytes act as barriers to lateral diffusion, but spectrin dimers seem to lack this function. Spectrin ∫ V is a non-erythrocytic member of the ∫-spectrin family. It is expressed in brain and pancreatic islets and localizes to the cytoplasm of the cytoskeleton and cell cortex. Spectrin ∫ V is a 2,564 amino acid protein with four isoforms due to alternative splicing events.

Subcellular Location:

Cytoplasm > cytoskeleton. Detected prominently in the outer segments of photoreceptor rods and cones and in the basolateral membrane and cytosol of gastric epithelial cells.

Tissue Specificity:

Expressed at very low levels in many tissues, with strongest expression in cerebellum, spinal cord, stomach, pituitary gland, liver, pancreas, salivary gland, kidney, bladder, and heart.

Similarity:

Belongs to the spectrin family.

Contains 2 CH (calponin-homology) domains.

Contains 1 PH domain.

Contains 31 spectrin repeats.

SWISS:

Q9NRC6

Gene ID:

51322

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.