产品货号 :  mlR5915

英文名称 :  MBD1

中文名称 :  甲基化CpG结合蛋白抗体

别    名 :  CXXC 3; CXXC3; MBD 1; MBD1; MECP1 COMPLEX; Methyl CpG binding domain protein 1; Methyl CpG binding domain protein 1 isoform PCM1; Methyl CpG binding protein MBD1; Methyl CpG binding protein splice variant 1; Methyl CpG binding protein splice variant 2; Methyl CpG binding protein splice variant 3; Methyl CpG binding protein splice variant 4; PCM 1; PCM1; Protein containing methyl CpG binding domain 1; Regulator of fibroblast growth factor 2 (FGF 2) transcription; RFT; The regulator of fibroblast growth factor 2(FGF 2) transcription; MBD1_HUMAN.  

研究领域 :  细胞生物  免疫学  染色质和核信号  细胞周期蛋白  细胞分化  

抗体来源 :  Rabbit

克隆类型 :  Polyclonal

交叉反应 :   Human, Mouse, Rat,

产品应用 :  WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  60kDa

细胞定位 :  细胞核

性    状 :  Lyophilized or Liquid

浓    度 :  1mg/ml

免 疫 原 :  KLH conjugated synthetic peptide derived from human MBD1:101-200/605

亚    型 :  IgG

纯化方法 :  affinity purified by Protein A

储 存 液 :  0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件 :  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed :  PubMed

产品介绍 :   DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract.

Subcellular Location:

Nucleus speckle. Chromosome.

Tissue Specificity:

Widely expressed.

Similarity:

Contains 3 CXXC-type zinc fingers.

Contains 1 MBD (methyl-CpG-binding) domain.

SWISS:

Q9UIS9

Gene ID:

4152

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片