产品货号 :  mlR33081

英文名称 :  AFP

中文名称 :  甲胎蛋白单克隆抗体

别    名 :  Alpha Fetoprotein; AFP; alpha 1 fetoprotein; alpha fetoglobulin; alpha Fetoprotein; Alpha fetoprotein precursor; FETA; HPAFP.  

研究领域 :  肿瘤  

抗体来源 :  Mouse

克隆类型 :  Monoclonal

克 隆 号 :  8D7

交叉反应 :   Human,

产品应用 :  IHC-P=1:500-1000 IHC-F=1:500-1000 ICC=1:100-500 IF=1:500-1000 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  70kDa

细胞定位 :  分泌型蛋白

性    状 :  Lyophilized or Liquid

浓    度 :  1mg/ml

免 疫 原 :  purifide human alpha 1 Fetoprotein:

亚    型 :  IgG

纯化方法 :  affinity purified by Protein G

储 存 液 :  0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件 :  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed :  PubMed

产品介绍 :   Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries.

Subunit:

Dimeric and trimeric forms have been found in addition to the monomeric form.

Subcellular Location:

Secreted.

Tissue Specificity:

Plasma. Synthesized by the fetal liver and yolk sac.

Similarity:

Belongs to the ALB/AFP/VDB family.

Contains 3 albumin domains.

SWISS:

P02771

Gene ID:

174

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

AFP是由胚胎卵黄囊细胞，胚胎肝细胞和胎儿肠道细胞合成的一种糖蛋白。主要用于原发性或转移性肝细胞癌、肝样胃癌和性腺或性腺外某些生殖细胞肿瘤(如内胚窦瘤)的诊断和鉴别诊断。

AFP敏感度较低，特异性强，大约60%左右的肝细胞肝癌阳性，在低分化肿瘤中常呈阳性表达。

产品图片