产品货号 :  mlR4730

英文名称 :  NADPH oxidase 4

中文名称 :  NADPH氧化酶4抗体

别  名 :  Kidney oxidase-1; Kidney superoxide-producing NADPH oxidase; KOX 1; KOX; Kox-1; NADPH; NADPH oxidase 4; Nox4; NOX4_HUMAN; Renal NAD(P)H-oxidase; RENOX.  

研究领域 :  肿瘤  心血管  细胞生物  免疫学  信号转导  新陈代谢  

抗体来源 :  Rabbit

克隆类型 :  Polyclonal

交叉反应 :   Human,

产品应用 :  WB=1:500-2000 ELISA=1:500-1000  
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  67kDa

细胞定位 :  细胞膜

性    状 :  Lyophilized or Liquid

浓    度 :  1mg/ml

免 疫 原 :  KLH conjugated synthetic peptide derived from human NOX4:201-300/578 <Extracellular>

亚    型 :  IgG

纯化方法 :  affinity purified by Protein A

储 存 液 :  0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件 :  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed :  PubMed

产品介绍 :   Nox4 is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. Nox4 is also expressed in fetal tissues, placenta, glioblastoma and vascular cells. Like gp91-phox, the enzymatic activity of Nox4 produces superoxide anions. In vascular cells, the addition of angiotensin II increases Nox4 expression, which suggests a role for Nox-4 in vascular oxidative stress response.

Function:

Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 4 displays an increased activity. Isoform 5 and isoform 6 display reduced activity.

Subunit:

Interacts with protein disulfide isomerase (By similarity). Interacts with, relocalizes and stabilizes CYBA/p22phox. Interacts with TLR4.

Subcellular Location:

Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein (Probable). Cell junction, focal adhesion (Probable). Nucleus (Probable). Note=May localize to plasma membrane and focal adhesions. According to PubMed:15927447, may also localize to the nucleus.

Tissue Specificity:

Mainly expressed in heart and skeletal muscle. Also strongly expressed in fetal brain and spinal cord.

Post-translational modifications:

Isoform 3 and isoform 4 are N-glycosylated. Isoform 4 glycosylation is required for its proper function.

Similarity:

Contains 1 FAD-binding FR-type domain.

Contains 1 ferric oxidoreductase domain.

SWISS:

Q9NPH5

Gene ID:

50507

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

还原型辅酶烟酰胺腺嘌呤二核苷酸磷酸(NADPH)oxidase 4:还原型辅酶烟胺腺嘌呤二核苷酸（Nicotinamide adenine dinucleotide reduced, NADH）位于线粒体膜内，是细胞能量代谢所必需的辅酶，主要功能是经电子传递链产生ATP。NADH在维持细胞生长、分化和能量代谢中起重要的作用。

产品图片