产品货号 :  mlR9719

英文名称 :  PDE4A8

中文名称 :  磷酸二酯酶4A8抗体

别   名 :  2EL; DPDE2; PDE4; PDE46; PDE4A; Phosphodiesterase 4A; Phosphodiesterse Type 4A8; PDE4A_HUMAN.  

研究领域 :  细胞生物  信号转导  细胞周期蛋白  细胞分化  

抗体来源 :  Rabbit

克隆类型 :  Polyclonal

交叉反应 :   Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Sheep,

产品应用 :  ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:50-200 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  98kDa

细胞定位 :  细胞浆

性    状 :  Lyophilized or Liquid

浓    度 :  1mg/ml

免 疫 原 :  KLH conjugated synthetic peptide derived from human PDE4A8:201-300/886

亚    型 :  IgG

纯化方法 :  affinity purified by Protein A

储 存 液 :  0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件 :  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed :  PubMed

产品介绍background :  

The protein encoded by this gene belongs to the cyclicnucleotide phosphodiesterase (PDE) family, and PDE4 subfamily. ThisPDE hydrolyzes the second messenger, cAMP, which is a regulator andmediator of a number of cellular responses to extracellularsignals. Thus, by regulating the cellular concentration of cAMP,this protein plays a key role in many important physiologicalprocesses. Alternatively spliced transcript variants encodingdifferent isoforms have been described for this gene.[provided byRefSeq, Jul 2011].

Function:

Hydrolyzes the second messenger cAMP, which is a keyregulator of many important physiological processes.

Subunit:

Isoform 1 interacts with LYN. Isoform 2 and isoform 6interact weakly with LYN. Isoform 1, isoform 2 and isoform 6interact with ARRB2.

Subcellular Location:

Isoform 1: Cytoplasm, perinuclear region.

Tissue Specificity:

Isoform 1 is widely expressed. Isoform 2 isabundant in liver, stomach, testis, thyroid and adrenal glands. Itis also found in placenta, kidney, pancreas, ovary, uterus, skin,monocytes, mast cells, macrophages, as well as in bronchial smoothmuscle. Isoform 6 is expressed at high levels in the heart andsmall intestine. It is also found in the brain, kidney, spleen,colon, salivary gland, ovary and peripheral blood lymphocytes.Isoform 7 is expressed predominantly in skeletal muscle and brainand at lower levels in the testis. Isoform 7 is expressed in thebrain. Found in specific neuronal subpopulations in cortex, spinalcord and cerebellum (at protein level).

Post-translational modifications:

Phosphorylation by MAPKAPK2 its activation through PKAphosphorylation (By similarity). Phosphorylated at Ser-686 andSer-688 when expressed in S.frugiperda cells. Isoform 2 and isoform7 are activated by phosphorylation at Ser-119 and Ser-123respectively by PKA.

Proteolytically cleaved by caspase-3.

Similarity:

Belongs to the cyclic nucleotide phosphodiesterasefamily. PDE4 subfamily.

SWISS:

P27815

Gene ID:

5141

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.