产品货号 :  mlR3312

英文名称 :  Phospho-NuMA (Ser395)

中文名称 :  磷酸化核有丝分裂器NuMA蛋白抗体

别    名 :  NuMA (Phospho Ser395); NuMA (Phospho S395); p-NuMA (Ser395); NUMA 1; NuMA protein; NUMA1; SP H antigen; Structural nuclear protein; Centrophilin stabilizes mitotic spindle in mitotic cells; Nuclear mitotic apparatus protein 1; Nuclear mitotic apparatus protein; NUMA1_HUMAN; NMP 22; NMP22; Nuclear matrix protein 22; Nuclear mitotic apparatus protein 1; Nuclear mitotic apparatus protein; NuMA protein; SP-H antigen.  

产品类型 :  磷酸化抗体

研究领域 :  肿瘤  细胞生物  免疫学  染色质和核信号  神经生物学  

抗体来源 :  Rabbit

克隆类型 :  Polyclonal

交叉反应  :  Human,

产品应用 :  ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  238kDa

细胞定位 :  细胞核 细胞浆 细胞膜

性    状 :  Lyophilized or Liquid

浓    度 :  1mg/ml

免 疫 原 :  KLH conjugated Synthesised phosphopeptide derived from human NuMA around the phosphorylation site of Ser395:HL(p-S)QL

亚    型 :  IgG

纯化方法 :  affinity purified by Protein A

储 存 液 :  0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件 :  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed :  PubMed

产品介绍 :   NuMA (Nuclear Mitotic Apparatus Protein) is an intranuclear protein and present in nucleus during interphase. At the onset of mitosis, it redistributes from the nucleus to two centrosomal structures that later will become part of the mitotic spindle pole. After anaphase, the protein redistributes from the spindle polar region into reforming nucleus. NuMA is an essential protein during mitosis for the terminal phases of chromosome separation and/or nuclear reassembly. Recently a study shows that NuMA is cleaved to a 180 to 200kDa during apoptosis.

Function:

Highly abundant component of the nuclear matrix where it may serve a non-mitotic structural role, occupies the majority if the nuclear volume. Required for maintenance and establishment of the mitotic spindle poles, functionning as a tether linking bulk microtubules of the spindle to centrosomes. May be involved in coordination of the alignment of the mitotic spindle to the cellular polarity axis, which is a prerequisite for asymmetric cell divisions.

Subunit:

Homodimer. Also forms multiarm oligomers by association of C-terminal tail domains, oligomers may further assemble to form a hexagonal nuclear lattice-like network. Interacts with TNKS.

Subcellular Location:

Nucleus matrix. Chromosome. Cytoplasm, cytoskeleton, spindle pole. Note=Resides in the nuclear matrix during interphases. Dissociates from condensing chromosomes during early prophase, and relocates to the spindle poles via dynein/dynamin association, it remain there until the anaphase onset. Before the complete disintegration of the nuclear lamina. As mitosis progresses it reassociates with telophase chromosomes very early during nuclear reformation, before substantial accumulation of lamins on chromosomal surfaces is evident.

Isoform Numa-m: Cytoplasm. Note=Mainly clustered at the centrosomal region.

Isoform Numa-s: Cytoplasm. Note=Mainly clustered at the centrosomal region.

Post-translational modifications:

ADP-ribosylated by TNKS during mitosis.

Phosphorylated in the C-terminal tail during mitosis, probably by CDK1. Phosphorylation increases solubility and promotes association with dynein and subsequent translocation to the spindle poles.

SWISS:

Q14980

Gene ID:

4926

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

NuMA (Nuclear mitotic apparatus protein)是细胞核基质中的一种高分子量蛋白质，具有一个特殊的细胞周期依赖性.核有丝分裂器蛋白NuMA与人类肿瘤细胞的恶性增殖相关.