产品货号 :  mlR19202

英文名称 :  Nephronectin

中文名称 :  肾连蛋白抗体

别    名 :  EGFL6L; Nephronectin; NPNT; NPNT_HUMAN; POEM; Preosteoblast EGF like repeat protein with MAM domain; Preosteoblast EGF-like repeat protein with MAM domain; Protein EGFL6 like; Protein EGFL6-like.  

研究领域 :  细胞生物  发育生物学  信号转导  

抗体来源 :  Rabbit

克隆类型 :  Polyclonal

交叉反应 :  Human, Mouse, Rat, Rabbit,

产品应用 :  ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  59kDa

细胞定位 :  分泌型蛋白

性    状 :  Lyophilized or Liquid

浓    度 :  1mg/ml

免 疫 原 :  KLH conjugated synthetic peptide derived from human Nephronectin:501-565/565

亚    型 :  IgG

纯化方法 :  affinity purified by Protein A

储 存 液 :  0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件 :  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed :  PubMed

产品介绍 :   Nephronectin is a 565 amino acid member of the nephronectin family that contains five EGF-like domains and an Arg-Gly-Asp (RGD) cell binding motif, as well as a meprin, A5 protein and receptor protein-tyrosine phosphatase µ (MAM) domain. Expressed in kidney, thyroid and parathyroid glands, developing bone, tooth germ, skeletal and smooth muscle, brain and skin, POEM is involved in the development and function of bone, muscle and kidney tissue. The most established function of POEM is as a ligand to the receptor Integrin å8/∫1. Highly expressed in the nephritic cord, Integrin å8/∫1 plays a critical role in kidney morphogenesis and development. The POEM-Integrin å8∫1 complex regulates the expression of GDNF, another protein that affects kidney development, specifically by regulating the growth of the uretic bud. There are two named isoforms of POEM, both of which form homodimers or homotrimers.

Function:

Functional ligand of integrin alpha-8/beta-1 in kidney development. Regulates the expression of GDNF with integrin alpha-8/beta-1 which is essential for kidney development. May also play a role in the development and function of various tissues, regulating cell adhesion, spreading and survival through the binding of several integrins.

Subcellular Location:

Secreted > extracellular space > extracellular matrix. Trapped on the cell surface or in the extracellular matrix.

Tissue Specificity:

Expressed in kidney and lung and to a lower extent in brain, pregnant uterus, placenta, thyroid gland and blood vessels.

Similarity:

Belongs to the nephronectin family.

Contains 5 EGF-like domains.

Contains 1 MAM domain.

SWISS:

Q6UXI9

Gene ID:

255743

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.