产品货号 :  mlR20550

英文名称 :  GRK3

中文名称 :  锚蛋白重复域54抗体

别    名 :  ADRBK2; Adrenergic, beta, receptor kinase 2; ARBK2_HUMAN; BARK2; Beta adrenergic receptor kinase 2; Beta ARK 2; Beta-adrenergic receptor kinase 2; Beta-ARK-2; EC 2.7.11.15; G protein coupled receptor kinase 3; G-protein-coupled receptor kinase 3.  

研究领域 :  细胞生物  免疫学  转录调节因子  G蛋白偶联受体  表观遗传学  G蛋白信号  

抗体来源 :  Rabbit

克隆类型 :  Polyclonal

交叉反应 :   Human, Mouse, Rat, Chicken, Dog, Cow, Horse, Sheep,

产品应用 :  ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  80kDa

细胞定位 :  细胞核 细胞浆

性    状 :  Lyophilized or Liquid

浓    度 :  1mg/ml

免 疫 原 :  KLH conjugated synthetic peptide derived from human GRK3:601-688/688

亚    型 :  IgG

纯化方法 :  affinity purified by Protein A

储 存 液 :  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

保存条件 :  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed :  PubMed

产品介绍background:

Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist. This phenomenon, referred to as agonist-mediated desensitization, involves phosphorylation of the receptor by two classes of enzymes. The first class is comprised of the second messenger-regulated kinases, such as c-AMP dependent protein kinase A and protein kinase C. The second class includes the G protein-coupled receptor kinases (GRKs). At least seven members of the GRK family have been identified. These include rhodopsin kinase (GRK 1), two forms of Beta-adrenergic receptor kinase: GRK 2 (Beta ARK, Beta ARK1) and GRK 3 (Beta ARK2), IT-11 (GRK 4), GRK 5, GRK 6 and GRK 7. Phosphorylation of receptors by GRKs appears to be strictly dependent on the receptor being in its agonist-activated state.

Function:

Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors.

Similarity:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

GPRK subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 PH domain.

Contains 1 protein kinase domain.

Contains 1 RGS domain.

SWISS:

P35626

Gene ID:

157

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片