产品货号 :  mlR1802

英文名称 :  Moesin

中文名称 :  膜突蛋白抗体

别    名 :  Membrane organizing extension spike protein; Moesin/anaplastic lymphoma kinase fusion protein; MSN; MSN/ALK fusion.   

研究领域 :  肿瘤  细胞生物  

抗体来源 :  Rabbit

克隆类型 :  Polyclonal

交叉反应  :  Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit,

产品应用 :  WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  63kDa

细胞定位 :  细胞浆 细胞膜

性    状 :  Lyophilized or Liquid

浓    度 :  1mg/ml

免 疫 原 :  KLH conjugated synthetic peptide derived from human Moesin:151-250/577

亚    型 :  IgG

纯化方法 :  affinity purified by Protein A

储 存 液 :  0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件 :  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed :  PubMed

产品介绍 :   Moesin is a member of the ERM (Ezrin-Radixin-Moesin) protein family, which possesses structural and probably functional relationships; they function as plasma membrane-actin cytoskeleton linkers and participate in the formation of specialized domains of the plasma membrane and are all localized to the submembranous cytoskeleton.

Moesin is widely expressed in different tissues in cells, where it is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signalling and for cell movement.

Function:

Probably involved in connections of major cytoskeletal structures to the plasma membrane. May inhibit herpes simplex virus 1 infection at an early stage.

Subunit:

In resting T-cells, part of a PAG1-SLC9A3R1-MSN complex which is disrupted upon TCR activation. Binds SLC9A3R1. Interacts with PPP1R16B. Interacts with PDZD8. Interacts with SELPLG and SYK; mediates the activation of SYK by SELPLG.

Subcellular Location:

Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Apical cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, microvillus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Phosphorylated form is enriched in microvilli-like structures at apical membrane. Increased cell membrane localization of both phosphorylated and non-phosphorylated forms seen after thrombin treatment.

Tissue Specificity:

In all tissues and cultured cells studied.

Post-translational modifications:

Phosphorylation on Thr-558 is crucial for the formation of microvilli-like structures. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding (By similarity). Phosphorylation on Thr-558 by STK10 negatively regulates lymphocyte migration and polarization.

Similarity:

Contains 1 FERM domain.

SWISS:

P26038

Gene ID:

4478

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Moesin是细胞骨架与细胞膜之间的连接蛋白,对细胞膜区域的整合和稳定性起到结构和功能调控的作用.通过连接细胞骨架和细胞膜。Moesin对细胞黏附、形态、分化、运动等起到重要调节作用。膜突蛋白早先被鉴定为一种硫酸乙酰肝素的可能受体和细胞骨架的连接蛋白，能稳定细胞表面的微绒毛、丝足和层形足板。膜-细胞骨架连接蛋白的埃兹蛋白-根蛋白-膜突蛋白（ERM）家族的成员都具有与质膜和肌动蛋白细胞骨架分别相连的氨基端和羧基端结构域。