产品货号 : mlR0991
英文名称 : HPV18 E6 protein
中文名称 : 人类乳头状瘤病毒18 E6抗体
别 名 : E6; Human Papilloma Virus; Human papillomavirus type 18 E6; Human papillomavirus type 18 (E6); Human papillomavirus type 18 E6; Human papillomavirus type 18 E6 protein; Protein E6; HPV18 E6 protein; VE6_HPV18.
研究领域 : 肿瘤 细菌及病毒
抗体来源 : Rabbit
克隆类型 : Polyclonal
交叉反应 : Human, HPV18
产品应用 : ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 : 11kDa
性 状 : Lyophilized or Liquid
浓 度 : 1mg/ml
免 疫 原 : KLH conjugated synthetic peptide derived from HPV18 E6:75-158/158
亚 型 : IgG
纯化方法 : affinity purified by Protein A
储 存 液 : 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 : Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed : PubMed
产品介绍 : Human papilloma viruses (HPVs) can be classified as either high risk or low risk according to their association with cancer. HPV16 and HPV18 are the most common of the high risk group while HPV6 and HPV11 are among the low risk types. Approximately 90% of cervical cancers contain HPV DNA of the high risk types. Mutational analysis have shown that the E6 and E7 genes of the high risk HPVs are necessary and sufficient for HPV transforming function. The specific interactions of the E6 and E7 proteins with p53 and pRB, respectively, correlate with HPV high and low risk classifications. The high risk HPV E7 proteins bind to pRB with a higher affinity than do the low risk HPV proteins, and only the high risk HPV E6 proteins form detectable complexes with p53 in vitro.
Function:
Transcriptional transactivator. Binds double stranded DNA (By similarity). Has transforming activity. Inactivates, with E6-AP ubiquitin-protein ligase, the human p53/TP53 tumor suppressor protein by targeting it to degradation. Binds and targets human MUPP1/MPDZ protein to degradation. Those two functions presumably contribute to transforming activity. Interaction with human FBLN1 protein also seems to be linked to cell transformation.
Subunit:
Forms a complex with E6-AP ubiquitin-protein ligase which interacts with human P53. Binds to human FBLN1, MAGI3 and MPDZ.
Subcellular Location:
Host nucleus matrix (By similarity).
Similarity:
Belongs to the papillomaviridae E6 protein family.
SWISS:
P06463
Gene ID:
1489088
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
人类乳头状瘤病毒18（HPV18-E6）是导致女性宫颈癌的主要病毒型（包括人类乳头状瘤病毒16）
