产品货号 :  mlR2024

英文名称 :  HBsAg(H2F4)

中文名称 :  人乙型肝炎表面抗原单克隆抗体（检测）

别    名 :  Hepatitis B Virus Surface Antigen; HBsAg; HBV major surface antigen; HBV surface antigen; Hepatitis B Virus major surface antigen; Major surface antigen; S; HBSAG_HBVC5.  

研究领域 :  免疫学  细菌及病毒  

抗体来源 :  Mouse

克隆类型 :  Monoclonal

克 隆 号 :  H2F4

交叉反应  :  HBsAg

产品应用 :  ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  44kDa

细胞定位 :  细胞膜

性    状 :  Lyophilized or Liquid

浓    度 :  1mg/ml

免 疫 原 :  Human Hepatitis B Surface Antigen protein:

亚    型 :  IgG

纯化方法 :  affinity purified by Protein A

储 存 液 :  0.01M TBS(pH7.4) with 0.03% Proclin300

保存条件 :  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed :  PubMed

产品介绍 :   Hepatitis B Virus Surface Antigen, Hepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase.

Function:

The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assumes fusion between virion membrane and endosomal membrane (Probable). In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein.

The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid.

Subunit:

Li-HBsAg interacts with capsid protein and with HDV Large delta antigen. Isoform M associates with host chaperone CANX through its pre-S2 N glycan. This association may be essential for M proper secretion.

Subcellular Location:

Virion membrane.

Post-translational modifications:

Isoform M is N-terminaly acetylated at a ratio of 90%, and N-glycosylated at the pre-S2 region.

Myristoylated.

Similarity:

Belongs to the orthohepadnavirus major surface antigen family.

SWISS:

P03138

Gene ID:

944569

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

人乙型肝炎表面抗原抗体