产品货号 :  mlR7502

英文名称 :  GTP binding protein REM1

中文名称 :  GTP结合蛋白REM1抗体

别    名 :  Rad and Gem related GTP binding protein; GES; GTP binding protein REM; GTP binding protein REM 1; GTP binding protein REM1; GTPase GES; GTPase regulating endothelial cell sprouting; Rad and Gem like GTP binding protein 1; RAS (RAD and GEM) like GTP binding; RAS like GTP binding; RAS like GTP binding protein REM; REM 1; REM1; E030011C07Rik; RP23-35I8.9; GD:REM; MGC114556; MGC48669; OTTMUSP00000016855; REM1_HUMAN.  

研究领域 :  心血管  细胞生物  信号转导  生长因子和激素  转录调节因子  通道蛋白  血管内皮细胞  G蛋白信号  

抗体来源 :  Rabbit

克隆类型 :  Polyclonal

交叉反应 :   Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Sheep,

产品应用 :  WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  33kDa

细胞定位 :  细胞膜

性    状 :  Lyophilized or Liquid

浓    度 :  1mg/ml

免 疫 原 :  KLH conjugated synthetic peptide derived from human REM/GTP binding protein REM1:221-298/298

亚    型 :  IgG

纯化方法 :  affinity purified by Protein A

储 存 液 :  0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件 :  Store at -20 癈 for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20癈. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 癈.

PubMed :  PubMed

产品介绍 :   REM (Rad and Gem related GTP binding protein) is a member of the Rad/Gem/Kir subfamily of Ras-like GTPases and shares with other members of this subfamily some unusual structural features. Among these are nonconservative amino acid substitutions within guanine nucleotide binding and hydrolysis domains, unique effector domains, extended N- and C-termini, and a conserved C-terminal sequence thought to mediate membrane association but lacking a classical isoprenylation motif. REM, with a predicted molecular weight of 32.9 kDa, is most highly expressed in cardiac muscle and is expressed at more moderate levels in lung, kidney and skeletal muscle. REM is phosphorylated in vivo and has been shown to interact with several 14-3-3 isoforms. It has been reported that the GTP-bound form of a related Ras-like GTPase, GEM/kir, inhibits high-voltage activated Ca2+ channel activities by interacting directly with the ?subunit. The reduced channel activities are the result of a decreased a-subunit expression at the plasma membrane. This inhibition of L-type Ca2+ channels prevents Ca2+-triggered exocytosis in hormone-secreting cells. There are data that suggest that REM similarly regulates Ca2+ channel expression.

Function:

Promotes endothelial cell sprouting and actin cytoskeletal reorganization. May be involved in angiogenesis. May function in Ca(2+) signaling.

Subunit:

In vitro, interacts with calmodulin in a calcium-dependent manner.

Tissue Specificity:

Most highly expressed in the endothelial lining of the blood vessels in uterus and heart. Lower levels found in spleen, lymph node, kidney and testis. Also found in cells with secretory function such as the islets of Langerhans, lobule/duct epithelium in the breast, bile duct epithelium in the liver, surface epithelium in the endometrial glands of the uterus, colon mucosa and acinar cells in the pancreas and the prostate.

Similarity:

Belongs to the small GTPase superfamily. RGK family.

SWISS:

O75628

Gene ID:

28954

Database links:

UniProtKB/Swiss-Prot: O75628.2

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.