产品货号 :  mlR11659

英文名称 :  GRC5

中文名称 :  GRC5蛋白抗体

别    名 :  CENP-35; GRC5; PHF2; JHDM1E; Jumonji C domain containing histone demethylase 1E; jumonji containing protein; Lysine-specific demethylase PHF2; PHD finger protein 2; Phf2; PHF2_HUMAN.  

研究领域 :  细胞生物  神经生物学  信号转导  转录调节因子  表观遗传学  

抗体来源 :  Rabbit

克隆类型 :  Polyclonal

交叉反应 :   Human, Mouse, Rat, Chicken, Horse, Sheep,

产品应用 :  WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  121kDa

细胞定位 :  细胞核

性    状 :  Lyophilized or Liquid

浓    度 :  1mg/ml

免 疫 原 :  KLH conjugated synthetic peptide derived from human GRC5/PHF2:1001-1096/1096

亚    型 :  IgG

纯化方法 :  affinity purified by Protein A

储 存 液 :  0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件 :  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed :  PubMed

产品介绍 :   PHF2 is a 1,101 amino acid protein belonging to the PHD finger protein family. Members of the PHD finger protein family function as transcriptional regulators that affect gene expression by modulating chromatin structure. Expressed in a wide variety of tissues, PHF2 localizes to the nucleus and contains one PHD-type zinc finger and one JMJC domain, suggesting a possible role for PHF2 in transcription regulation and chromatin remodeling. The gene encoding PHF2 lies in the candidate region for hereditary sensory neuropathy type I (HSN1), a disorder characterized by sensory dysfunction. PHF2 exhists as two isoforms produced by alternative splicing, designated isoform 1 and 2 respectively.

Function:

Lysine demethylase that demethylates both histones and non-histone proteins. Enzymatically inactive by itself, and becomes active following phosphorylation by PKA: forms a complex with ARID5B and mediates demethylation of methylated ARID5B. Demethylation of ARID5B leads to target the PHF2-ARID5B complex to target promoters, where PHF2 mediates demethylation of dimethylated 'Lys-9' of histone H3 (H3K9me2), followed by transcription activation of target genes. The PHF2-ARID5B complex acts as a coactivator of HNF4A in liver. PHF2 is recruited to trimethylated 'Lys-4' of histone H3 (H3K4me3) at rDNA promoters and promotes expression of rDNA.

Subunit:

Component of the PHF2-ARID5B complex, at least composedof PHF2 and ARID5B. Interacts with HNF4A and NR1H4.

Subcellular Location:

Nucleus, nucleolus.

Tissue Specificity:

Widely expressed, including in liver (at protein level).

Post-translational modifications:

Phosphorylated by PKA on specific serine residues, leading to the formation of an active lysine demethylase complex.

Similarity:

Belongs to the JHDM1 histone demethylase family. JHDM1D subfamily.

Contains 1 JmjC domain.

Contains 1 PHD-type zinc finger.

SWISS:

O75151

Gene ID:

5253

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片