产品货号 :  mlR10765

英文名称 :  CSFV E2 protein

中文名称 :  猪瘟病毒E2蛋白抗体

别    名 :  CSFV Polyprotein; CSFV E2 protein; CSFV E2; polyprotein [Classical swine fever virus]; polyprotein; CSFV polyprotein; E2; Envelope glycoprotein E2; CSFV Envelope glycoprotein E2; Classical swine fever virus E2; Classical swine fever virus Envelope glycoprotein E2; structural protein E2 [Classical swine fever virus].  

研究领域 :  细菌及病毒  

抗体来源 :  Rabbit

克隆类型 :  Polyclonal

交叉反应 :  CSFV

产品应用 :  ELISA=1:500-1000  
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  42kDa

细胞定位 :  细胞膜

性    状 :  Lyophilized or Liquid

浓    度 :  1mg/ml

免 疫 原 :  Recombinded full length CSFV E2 protein:full length

亚    型 :  IgG

纯化方法 :  affinity purified by Protein A

储 存 液 :  0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件 :  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed :  PubMed

产品介绍 :  Classical swine fever(CSF) is a kind of acute and intense contagious disease in swine, it material damages swine industry. CSF could cause swine appearing disseminated hemorrhage in systemic organ and tissue, degeneration and necrosis in small vascular and capillary endothelial cells. Classical swine fever virus (CSFV) belongs to genera Pestivirus, family Flaviviridae, it’s genome total length is 12.3Kb,including a big ORF, encoding 11 structural and nonstructural proteins.

Function:

E(rns), E1 and E2 are responsible of cell attachment and subsequent fusion of viral and cellular membrane.

P7 forms a leader sequence to properly orient NS2 in the membrane.

Uncleaved NS2-3 is required for production of infectious virus.

NS2 protease seems to play a vital role in viral RNA replication control and in the pathogenicity of the virus.

NS3 displays three enzymatic activities: serine protease, NTPase and RNA helicase.

NS4A is a cofactor for the NS3 protease activity.

RNA-directed RNA polymerase NS5 replicates the viral (+) and (-) genome.

Subunit:

The E(rns) glycoprotein is found as a homodimer; disulfide-linked. The E1 and E2 envelope glycoproteins form disulfide-linked homodimers as well as heterodimers.

Subcellular Location:

Host cell surface.

Post-translational modifications:

The E(rns) glycoprotein is heavily glycosylated.

The viral RNA of pestiviruses is expressed as a single polyprotein which undergoes post-translational proteolytic processing resulting in the production of at least eleven individual proteins. The N-terminal protease cleaves itself from the nascent polyprotein autocatalytically and thereby generates the N-terminus of the adjacent viral capsid protein C.

Cleavage between E2 and p7 is partial.

Similarity:

Belongs to the pestivirus polyprotein family.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Contains 1 peptidase C53 domain.

Contains 1 peptidase C74 domain.

Contains 1 peptidase S31 domain.

Contains 1 RdRp catalytic domain.

SWISS:

P1971

Gene ID:

920146

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.