产品货号 :  mlR0212

英文名称 :  Hepatitis E Virus ORF3

中文名称 :  戊型肝炎病毒抗体

别    名 :  structural protein; immunogenic protein; ORF-3; ORF 3; Protein ORF3; pORF3; phosphoprotein; ORF3 [Hepatitis E virus]; ORF3_HEVHY.  

研究领域 :  免疫学  细菌及病毒  

抗体来源 :  Rabbit

克隆类型 :  Polyclonal

交叉反应  :  HEV

产品应用 :  WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

分 子 量 :  13.5kDa

性    状 :  Lyophilized or Liquid

浓    度 :  1mg/ml

免 疫 原 :  KLH conjugated synthetic peptide derived from HEV ORF3:

亚    型 :  IgG

纯化方法 :  affinity purified by Protein A

储 存 液 :  0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件 :  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMed :  PubMed

产品介绍 :   The Hepatitis E virus is the causative agent of Hepatitis E. Its taxonomic name is Orthohepevirus A. The viral genome a single-strand of positive-sense RNA that is approximately 7200 bases in length. It encodes 3 proteins (O1, O2, O3), two of which are polyproteins, that is, they are cleaved into fragments which carry out the actual functions of the virus. The O1 protein consists of 7 such fragments, namely Met (Methyltransferase), Y (Y-domain), Plp (Papain like protease), V (proline-rich variable region), X (X-domain, macro-domain), Hel (Helicase), and Rdrp (RNA dependent RNA polymerase). The Pvx domain is a fusion protein consisting of the Plp, V and X domains. The O3 protein is encoded by a single open reading frame (ORF3). The O2 protein encodes the capsid, which is composed of 3 domains, namely the shell domain (S) and two protruding domains (P1, P2). Numbers in the figure indicate positions in the RNA sequence.

Function:

May act as a viral regulatory protein involved in the modulation of mitogenic signaling pathways. May be involved in virion morphogenesis and viral pathogenesis. Expedites the processing and secretion of AMBP from the hepatocyte.

Subunit:

Homodimer. Interacts with host SRC, HCK, FYN, PIK3R3 and GRB2 (via SH3 domain); binding does not activate the kinases. Interacts with host AMBP/bikunin and AMBP/alpha-1-microglobulin peptides. Interacts with host HPX/hemopexin. The phosphorylated form interacts with the unglycosylated capsid protein.

Subcellular Location:

Host cytoplasm, host cytoskeleton. Note=The N-terminal region seems to associate with the cytoskeleton probably via one of its hydrophobic regions.

Post-translational modifications:

Not glycosylated.

Similarity:

Belongs to the hepevirus ORF3 protein family.

SWISS:

O90299

Gene ID:

N/A

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.