产品货号 : mlR33318
英文名称 : Firefly Luciferase
中文名称 : 荧光素酶单克隆抗体
别 名 : ec 1.13.12.7; Firefly; Firefly Luciferase Luciferase; Luciferin 4 monooxygenase.
产品类型 : 标签抗体
研究领域 : 激酶和磷酸酶 昆虫
抗体来源 : Mouse
克隆类型 : Monoclonal
克 隆 号 : 11D6
交叉反应 : Fruit Fly,
产品应用 : WB=1:500-5000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量 : 61kDa
细胞定位 : 细胞浆
性 状 : Lyophilized or Liquid
浓 度 : 1mg/ml
免 疫 原 : KLH conjugated synthetic peptide derived from human Firefly Luciferase:
亚 型 : IgG1
纯化方法 : affinity purified by Protein G
储 存 液 : 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件 : Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed : PubMed
产品介绍 : Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
Function:
Produces green light with a wavelength of 562 nm.
Subcellular Location:
Peroxisome.
Similarity:
Belongs to the ATP-dependent AMP-binding enzyme family.
SWISS:
N/A
Gene ID:
N/A
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品图片