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菌株名称 |
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菌株品牌 |
酶联生物 |
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基 因 型 |
F– ompT hsdSB(rB– mB–)gal dcm lacY1(DE3)pLysS (CamR) |
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菌株抗性 |
Chl |
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培 养 基 |
LB |
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菌株类别 |
大肠杆菌 |
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培养条件 |
37℃，有氧，LB |
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质粒转化 |
42℃热激 |
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保存方式 |
20%甘油，-20℃ |
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基本应用 |
用于基因克隆 |
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菌株简介 |
Tuner strains are lacZY deletion mutants of BL21 and enable adjustable levels of protein expression throughout all cells in a culture. The lac permease (lacY) mutation allows uniform entry of IPTG into all cells in the population, which produces a concentration-dependent, homogeneous level of induction. By adjusting the concentration of IPTG, expression can be regulated from very low levels up to the robust, fully induced levels commonly associated with pET vectors. Lower-level expression may enhance the solubility and activity of difficult target proteins. DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability. |
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供应范围 |
仅供限于科研实验研究使用 |
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运输方式 |
低温快递运输 |
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菌株优势 |
酶联生物菌株库拥有200种菌株 , 遗传性状一致 |
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特别说明 |
细胞培养/动物血清/实验服务/原代提取/菌株购买，请立即与酶联生物联系 |
