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菌株名称 |
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菌株品牌 |
酶联生物 |
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菌株抗性 |
kan /tet/Chl |
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培 养 基 |
LB |
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菌株类别 |
大肠杆菌 |
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培养条件 |
37℃，有氧，LB |
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质粒转化 |
42℃热激 |
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保存方式 |
20%甘油，-20℃ |
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基本应用 |
用于蛋白表达 |
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菌株简介 |
Origami B host strains carry the same mutations in trxB and gor as the original Origami strains, except that they are derived from a lacZY mutant of BL21 to enable precise control of expression levels by adjusting the concentration of IPTG. Thus the Origami B strains combine the desirable characteristics of BL21, Tuner™, and Origami strains in one strain background. The mutations in trxB and gor are selectable on kanamycin and tetracycline, respectively; therefore, these strains cannot be used with plasmids that can only selected with kanamycin or tetracycline. These strains also include the lon and ompT deficiences of BL21, which increase protein stability. DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG. pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability. |
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供应范围 |
仅供限于科研实验研究使用 |
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运输方式 |
低温快递运输 |
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菌株优势 |
酶联生物菌株库拥有200种菌株 , 遗传性状一致 |
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特别说明 |
细胞培养/动物血清/实验服务/原代提取/菌株购买，请立即与酶联生物联系 |
