Characteristics:
Source Chinese Hamster Ovary cell line
Description Human EPO (Ala28-Arg193) Accession # P01588
Predicted molecular 18.3 kDa
mass
Specification:
Appearance White powder, Colorless clear liquid after reconstitution
Purity ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin ≤10 EU/mg by the LAL method
Activity Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is
≤0.1 ng/mL, corresponding to a specific activity of ≥ 1.5 x 105 IU/mg.
≤0.1 ng/mL, corresponding to a specific activity of ≥ 1.5 x 105 IU/mg.
Formulation Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4
Size 10 μg/vial,50 μg/vial, 1 mg/vial
Handling and Storage:
Reconstitution It is recommended to redissolve in sterile deionized water.
Shipping The product is shipped with blue ice
Storage & Stability 36 months at -20°C to -80°C in lyophilized state 6 months at -20°C to -80°C under sterile
conditions after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
conditions after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Bioactivity:.jpg)
Recombinant Human EPO (Cat. No.mly05801) stimulates proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is ≤0.1 ng/mL.
