Characteristics:
Source Chinese Hamster Ovary cell line
Description Mouse GM-CSF(Ala18-Lys141) Accession # Q14AD9
Predicted molecular 14.3 kDa
Mass
Specification:
Appearance White powder,Colorless clear liquid after reconstitution
Purity ≥95%, by SDS-PAGE (under conditions, visualized by Coomassie staining)
Endotoxin ≤10 EU/mg by the LAL method
Activity The activity is determined by the dose-dependent proliferation of murine
NFS-60 cells is＜0.05 ng/ml, corresponding to a specific activity of ≥ 2 E7
units/mg.
Formulation Lyophilized from a 0.22 μm-filtered solution containing PBS,5% Mannitol
and 0.01% Tween 80, pH7.4
Size 50 μg/vial, 1 mg/vial
Handling and Storage:
Reconstitution It is recommended to redissolve in sterile deionized water.
Shipping The product is shipped with blue ice
Storage & Stability 36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution
7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Bioactivity:
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Measured in a cell proliferation assay using M-NFS-60 cells.
