Characteristics:
Source Chinese Hamster Ovary cell line
Description Human FLT-3L (Thr27-Pro184 Accession # P49771
Predicted molecular 17.8 kDa
Mass
Specification:
Appearance White powder, Colorless clear liquid after reconstitution
Purity ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin ≤10 EU/mg by the LAL method
Activity The ED50 as determined by the dose-dependent stimulation of theproliferation of human OCI-AML5 cells
is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.
is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 106 units/mg.
Formulation Lyophilized from a 0.22 μm filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4
Size 50 μg/vial, 1 mg/vial
Handling and Storage:
Reconstitution It is recommended to redissolve in sterile deionized water.
Shipping The product is shipped with blue ice
Storage & Stability 36 months at -20°C to -80°C in lyophilized state 7-10 days at 2°C to 8°C under sterile conditions
after reconstitution 6 months at -20°C to -80°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Bioactivity:
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Recombinant Human FLT-3L(Cat. No. mly01201 ) stimulates proliferation of OCI-AML5 cells. The ED50 for this effect is ≤1.0 ng/mL. The activity of recombinant Human FLT-3L(Cat. No. mly01201 ) was higher than the other competing product.
