Recombinant Human BMP-7
中文名：重组人骨髓基质蛋白7
Characteristics:
Source Chinese Hamster Ovary cell line
Description Human BMP-7 (Met316-His431) Accession # P18075
Predicted molecular 13.1 kDa (monomer）
Mass
Specification:
Appearance White powder, Colorless clear liquid after reconstitution
Purity ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions,
visualized by Coomassie staining)
Endotoxin ≤10 EU/mg by the LAL method
Activity Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells.
The expected ED50 for this effect is 0.02-0.04 μg/ml.
Formulation Lyophilized from a 0.22 μm-filtered solution containing 0.085% TFA, 30% ACN,5%
mannitol Trehalose, pH 3.0
mannitol Trehalose, pH 3.0
Size 50 μg/vial, 1 mg/vial
Handling and Storage:
Reconstitution It is recommended to redissolve in 4 mM HCl
Shipping The product is shipped with blue ice.
Storage & Stability 36 months at -20°C to -80°C in lyophilized state6 months at -20°C to -80°C under sterile
conditions after reconstitution 7-10days at 2°C to 8°C under sterile conditions after reconstitutionUse
a manual defrost freezer and avoid repeated freeze-thaw cycles.
Bioactivity:
.jpg)
Measured alkaline phosphatase production by ATDC-5 chondrogenic cells. The activity of mly00601 was higher than the other competing product.
