Characteristics:
Source Chinese Hamster Ovary cell line
Description Human Interleukin-4 (His25-Ser153)
Accession # P05112
Predicted molecular 15 kDa
Mass
Specification:
Appearance White powder, Colorless clear liquid after reconstitution
Purity ≥95%, by SDS-PAGE (under reducing (R) condition, visualized by
Coomassie staining)
Endotoxin ≤10 EU/mg by the LAL method
Activity Measured in a cell proliferation assay using TF-1 cells, The ED50 for this
effect is ≤ 0.2 ng/mL ，corresponding to a specific activity of ＞ 1x.
107 units/mg.
Formulation Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol
and 0.01% Tween 80, pH 7.4
Size 50 μg/vial, 1 mg/vial
Handling and Storage:
Reconstitution It is recommended to redissolve in sterile deionized water.
Shipping The product is shipped with blue ice.
Storage & Stability 36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution
7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Bioactivity:
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Measured in a cell proliferation assay using TF-1 cells.
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Highly consistent bioactivity between different production batches of Recombinant Human IL-4 (Cat. No. mly02201) .
mly02201 stimulates proliferation of TF-1 cells. The ED50 for this effect is ≤0.2 ng/mL.
