Characteristics:
Source Chinese Hamster Ovary cell line
Description Human HGF (α chain： Gln 32 -Arg 494 & β chain： Val 495-Ser 728) Accession # P14210
Predicted molecular 53.7 kDa ( α chain), 26 kDa ( β chain)
Mass
Specification:
Appearance White powder, Colorless clear liquid after reconstitution
Purity ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin ≤10 EU/mg by the LAL method
Activity Measured by its ability to induce IL-11 secretion by Saos-2 human osteosarcoma cells.
The ED50 for this effect is ≤2.5 ng/ml.
The ED50 for this effect is ≤2.5 ng/ml.
Formulation Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4
Size 50 μg/vial, 1 mg/vial
Handling and Storage:
Reconstitution It is recommended to redissolve in sterile deionized water
Shipping The product is shipped with blue ice.
Storage & Stability 36 months at -20°C to -80°C in lyophilized state 6 months at -20°C to -80°C under sterile conditions
after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Bioactivity:
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Measured by its ability to induce IL-11 secretion by Saos- 2 human osteosarcoma cells. The ED50 for this effect is ≤2.5 ng/ml. The activity of mly01701 was higher than the other competing product.
