Characteristics:
Source Chinese Hamster Ovary cell line
Description Human Transferrin (Val20-Pro698) Accession # P02787
Predicted molecular 75.2 kDa
Mass
Specification:
Appearance Red powder, red clear liquid after reconstitution
Purity ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin ≤0.5 EU/mg by the LAL method
Activity Measured in a serum-free cell proliferation assay using MDCK canine kidney epithelial cells.
The ED50 for this effect is 0.5-2 μg/mL.
Optimal concentration depends on cell type as well as the application or research objectives.
Formulation Lyophilized from a 0.22 μm filtered solution in sterile water.
Size 100 mg/vial, 1 g/vial, 10 g/vial, 100 g/vial
Handling and Storage:
Reconstitution Iis recommended to redissolve in sterile deionized water.
Shipping The product is shipped with blue ice
Storage & Stability 36 months at 2°C to 8°C in lyophilized stateunder sterile conditions
6 months at -20°C to -80°C under sterile conditions after reconstitution
7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Bioactivity:
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Recombinant Human Transferrin (Cat. No. mly04501) stimulates proliferation of MDCK cells in serum-free cell culture medium. The ED50 for this effect is 0.5-2μg/mL. The activity of mly04501 was higher than other competing products.
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Highly consistent bioactivity between different production batches of Recombinant Human Transferrin (Cat. No. mly04501) .
