Recombinant Human TNFα

Recombinant Human TNFα
           中文名：重组人tnfα 
 

Characteristics: 

 

Source                     Chinese Hamster Ovary cell line

 

Description               Human TNFα (Val77-Leu233)  Accession # P01375

 

Predicted molecular     17.4 kDa

Mass

Specification:

 

Appearance               White powder, Colorless clear liquid after reconstitution

 

Purity                          ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions,

                                   visualized by Coomassie staining)

 

Endotoxin                   ≤10 EU/mg by the LAL method

 

Activity                       Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the

                                   presence of the metabolic inhibitor actinomycin D. The ED50 for this effect

                                   is 5-60 pg/mL.

 

Formulation                Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol  

                                   and 0.01% Tween 80, pH 7.4

 

Size                            50 μg/vial, 1 mg/vial

 

Handling and Storage:

 

Reconstitution             It is recommended to redissolve in sterile deionized water.

 

Shipping                      The product is shipped with blue ice.

 

Storage & Stability       36 months at -20°C to -80°C in lyophilized state

                                     6 months at -20°C to -80°C under sterile conditions after reconstitution

                                     7-10 days at 2°C to 8°C under sterile conditions after reconstitution

                                     Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

Bioactivity: