Characteristics:
Source Chinese Hamster Ovary cell line
Description Human Transferrin (Val20-Pro698) Accession # P02787
Predicted molecular 75.2 kDa
Mass
Specification:
Appearance White powder, Colorless to light red solution with no opalescence after reconstitution
Purity ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin ≤10 EU/mg by the LAL method
Activity Measured in a serum-free cell proliferation assay using MDCK caninekidney epithelial cells. The ED50for
this effect is ≤5 μg/mL. Optimal.concentration depends on cell type as well as
the application or research objectives..
this effect is ≤5 μg/mL. Optimal.concentration depends on cell type as well as
the application or research objectives..
Formulation Lyophilized from a 0.22 μm filtered solution in sterile water.
Size 50 μg/vial, 1 mg/vial
Handling and Storage:
Reconstitution It is recommended to redissolve in sterile deionized water.
Shipping The product is shipped with blue ice.
Storage & Stability 36 months at 2°C to 8°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution
7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Bioactivity:
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Measured in a serum-free cell proliferation assay using MDCK canine kidney epithelial cells. The ED50 for this effect is ≤5 μg/mL. The activity of mly04504 was higher than other competing products.
