Recombinant Human DKK-1

Recombinant Human DKK-1
           中文名：重组人Dkk-1

Characteristics: 

 

Source                      Chinese Hamster Ovary cell line

 

Description                Human Dkk-1 (Thr32-His266)  Accession # O94907

 

Predicted molecular      25.7kDa

Mass

Specification:

 

Appearance               White powder, Colorless clear liquid after reconstitution

 

Purity                          ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions,

                                   visualized by Coomassie staining)

 

Endotoxin                   ≤10 EU/mg by the LAL method

 

Activity                        Measured by its ability to inhibit 50ng/ml wnta-3a induced Topflash

                                    reporter activity in CHO cells. The ED50  is  ≤50ng/ml.

 

Formulation                 Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol  

                                    and 0.01% Tween 80, pH 7.4

 

Size                             50 μg/vial, 1 mg/vial

 

Handling and Storage:

 

Reconstitution              It is recommended to redissolve in sterile deionized water.

 

Shipping                       The product is shipped with blue ice.

 

Storage & Stability       36 months at -20°C to -80°C in lyophilized state

                                     6 months at -20°C to -80°C under sterile conditions after reconstitution

                                     7-10 days at 2°C to 8°C under sterile conditions after reconstitution

                                     Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

Bioactivity:

 

Measured by its ability to inhibit 50ng/ml Wnt-3a induced Top flash reporter activity in CHO cells.