Characteristics:
Source Chinese Hamster Ovary cell line
Description Recombinant human IL-12 is composed of P40(Ile23-Ser328) and
P35(Arg23-Ser219) subunits,which linked by GS-linker（GSGSSRGGSGSGGSGGGGSK）
Accession # P29460(IL12P40), P29459(IL12P35)
Predicted molecular 59 kDa
Mass
Specification:
Appearance White powder, Colorless clear liquid after reconstitution
Purity ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions
visualized by Coomassie staining)
Endotoxin ≤10 EU/mg by the LAL method
Activity Measured by its ability to induce Interferon gamma secretion by human
natural killer lymphoma NK-92 cells. The ED50 for this effect is ＜0.5
ng/mL.
Formulation Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol
and 0.01% Tween 80, pH 7.4
Size 50 μg/vial, 1 mg/via
Handling and Storage:
Reconstitution
Shipping It is recommended to redissolve in sterile deionized water.
The product is shipped with blue ice.
Storage & Stability 36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution
7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Bioactivity: .jpg)
Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells.
The ED50 for this effect is ＜0.5 ng/mL. The activity of mly04001 was higher than other competing products.
