Recombinant Human Fetuin A

 

Recombinant Human Fetuin A
        中文名：重组人胎牛血清蛋白A

 

Characteristics: 

 

Source                          Chinese Hamster Ovary cell line

 

Description                    Human Fetuin A (A-Chain: A19-L300; B-Chain: T341-V367)  Accession # P02765

 

Predicted molecular      32.9 KDa

Mass

Specification:

 

Appearance                    White powder, Colorless clear liquid after reconstitution

 

Purity                              ≥95%,  by  SDS-PAGE  (under  reducing  (R)  condition,  visualized by

                                       Coomassie staining)

 

Endotoxin                       ≤0.5 EU/mg by the LAL method

 

Activity                            Measured in a cell proliferation assay using B16-F1 cells. Human Fetuin A

                                       (Cat. No. mly07501) stimulates adhesion of B16-F1 cells. The ED50 fo
     this effect is 50-200 μg/mL.
     Optimal concentration depends on cell type as well as the application or
     research objectives.

 

Formulation                     Lyophilized from a 0.22 μm-filtered solution containing 10 mM PB, pH 7.4

 

Size                                 100 mg/vial, 1g/vial, 10g/vial, 100g/vial

 

Handling and Storage:

 

Reconstitution                 It is recommended to redissolve in sterile deionized water.

 

Shipping                          The product is shipped with blue ice.

 

Storage & Stability          36 months at 2°C to 8°C in lyophilized state

                                        6 months at -20°C to -80°C under sterile conditions after reconstitution

                                        7-10 days at 2°C to 8°C under sterile conditions after reconstitution

                                        Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

Bioactivity: