Recombinant Human Noggin
中文名：重组人Noggin
Characteristics:
Source Chinese Hamster Ovary cell line
Description Human Noggin (Gln28-Cys232) Accession # Q13253
Predicted molecular 23 kDa
mass
Specification:
Appearance White powder, Colorless clear liquid after reconstitution
Purity ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions, visualized by Coomassie staining)
Endotoxin ≤10 EU/mg by the LAL method
Activity Determined by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by
ATDC-5 chondrogenic cells.The expected ED50 for this effect is 2.0-3.0 ng/ml of Noggin.
Formulation Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol and 0.01% Tween 80, pH 7.4
Size 50 μg/vial, 1 mg/vial
Handling and Storage:
Reconstitution It is recommended to redissolve in sterile deionized water.
Shipping The product is shipped with blue ice.
Storage & Stability 36 months at -20°C to -80°C in lyophilized state 6 months at -20°C to -80°C under sterile conditions
after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Bioactivity:
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Measured by its ability to inhibit 5.0 ng/mlof BMP-4 induced alkaline phosphatase production by ATDC-5 chondrocenic cells. The activity of Recombinant Human Noggin （ Cat mly03301） is equivalent to competitor.
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