Characteristics:
Source Chinese Hamster Ovary cell line
Description Human Vitronectin (Asp20-Leu478)
Accession #P04004
Predicted molecular 52.3kDa
Mass
Specification:
Appearance White powder, Colorless clear liquid after reconstitution
Purity ≥95%, by SDS-PAGE (under reducing (R) & Non-reducing conditions,
visualized by Coomassie staining)
visualized by Coomassie staining)
Endotoxin ≤1 EU/mg by the LAL method
Activity Measured by the ability of the immobilized protein to support the adhesion
of NIH3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 2-10
μg/mL.
Optimal concentration depends on cell type as well as the application or
research objectives.
of NIH3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 2-10
μg/mL.
Optimal concentration depends on cell type as well as the application or
research objectives.
Formulation Lyophilized from a 0.22 μm-filtered solution containing PBS, 5% mannitol
and 0.01% Tween 80, pH 7.4
and 0.01% Tween 80, pH 7.4
Size 50 μg/vial, 1 mg/vial
Handling and Storage:
Reconstitution It is recommended to redissolve in sterile deionized water.
Shipping The product is shipped with blue ice.
Storage & Stability 36 months at -20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution
7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Bioactivity:
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Measured by the ability of the immobilized protein to support the adhesion of NIH3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 2-10 μg/mL. The activity of mly04801L was higher than other competing products.
