Recombinant Bovine Fetuin A

Recombinant Bovine Fetuin A
           中文名：重组牛胎球蛋白A

Characteristics: 

 

Source                         Chinese Hamster Ovary cell line

 

Description                   Bovine Fetuin A (I19-I359)  Accession # P12763

 

Predicted molecular     36.4 KDa

Mass

Specification:

 

Appearance                   White powder, Colorless clear liquid after reconstitution

 

Purity                              ≥95%,  by  SDS-PAGE (under  reducing (R) condition,  visualized  by

                                       Coomassie staining)

 

Endotoxin                       ≤0.5 EU/mg by the LAL method

 

Activity                            Measured in a cell proliferation assay using B16-F1 cells. Recombinant

                                        Bovine Fetuin A (Cat. No. mly07511) stimulates adhesion of B16-F1
    cells. The ED50 for this effect is ≤100 μg/mL.
    Optimal concentration depends on cell type as well as the application or
     research objectives.

 

Formulation                     Lyophilized from a 0.22 μm-filtered solution containing 10 mM PB, pH 7.4

 

Size                                 100 mg/vial, 1g/vial, 10g/vial, 100g/vial

 

Handling and Storage:

 

Reconstitution                 It is recommended to redissolve in sterile deionized water.

 

Shipping                          The product is shipped with blue ice

 

Storage & Stability          36 months at 2°C to 8°C in lyophilized state

                                        6 months at -20°C to -80°C under sterile conditions after reconstitution

                                        7-10 days at 2°C to 8°C under sterile conditions after reconstitution

                                        Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

 

Bioactivity:

 

Recombinant  Bovine  Fetuin  A  (Cat.  No.  mly07511)  stimulates adhesion of B16-F1  cells. The  ED50   for  this  effect  is   ≤ 100 μg/mL.