Characteristics:
Source E. coli
Description Bovine bFGF (P10-S155) Accession # P03969
Predicted molecular 16.5 kDa
Mass
Specification:
Appearance White powder,Colorless clear liquid after reconstitution
Purity ≥95%, by SDS-PAGE (under reducing (R)&Non-reducing conditions,visualized by Coomassie staining)
Endotoxin ≤200 EU/mg by the LAL method
Activity Measured in a cell proliferation assay using NIH3T3 mouse embryonic fibroblast cells. The ED50 for this effect
is ≤2.0 ng/mL
is ≤2.0 ng/mL
Formulation Lyophilized from a 0.22 μm-filtered solution containing 10mMPB, 250mM NaCl,5%mannitol and 0.01% Tween
80 ,pH 7.4
80 ,pH 7.4
Size 50 μg/vial, 1 mg/vial
Handling and Storage:
Reconstitution It is recommended to redissolve in sterile deionized water.
Shipping The product is shipped with blue ice
Storage & Stability 36 months at- 20°C to -80°C in lyophilized state
6 months at -20°C to -80°C under sterile conditions after reconstitution
7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months at -20°C to -80°C under sterile conditions after reconstitution
7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Bioactivity:
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Measured in a cell proliferation assay using NIH3T3 cells.
