Characteristics:
Source Chinese Hamster Ovary cell line
Description Mouse Noggin (Q28-C232) Accession # P97466
Predicted molecular 23. 1kDa
mass
Specification:
Appearance White powder,Colorless clear liquid after reconstitution
Purity ≥95%, by SDS-PAGE (under reducing (R)&Non-reducing conditions,visualized by Coomassie staining)
Endotoxin ≤10 EU/mg by the LAL method
Activity Measured by its ability to inhibit 5.0ng/ml of BMP-4 induced alkaline
phosphatase production by ATDC-5 chondrogenic cells. The ED50 is ≤0. 1μ g/ml.
Formulation Lyophilized from a 0.22 μm filtered solution containing PBS,5% mannitol and 0.01% Tween 80, pH7.4
Size 50 μg/vial, 1 mg/vial
Handling and Storage:
Reconstitution It is recommended to redissolve in sterile deionized water.
Shipping The product is shipped with blue ice
Storage & Stability 36 months at -20°C to -80°C in lyophilized state 6 months at -20°C to -80°C under sterile conditions
after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution
after reconstitution 7-10 days at 2°C to 8°C under sterile conditions after reconstitution
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Bioactivity:
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Measured by its ability to inhibit 5.0 ng/ml of BMP-4 induced alkaline phosphatase production by ATDC-5 chondrogenic cells.
